骨髓间充质干细胞对大鼠心肌梗死后心肌营养素-1的影响  

Effect of bone marrow mesenchymal stem cells transplantation on the expression of cardiotrophin-1 in ventricular remodeling after acute myocardial infarction in rats

作　　者：李恩[1] 牛少辉[1] 孙利强[1] 刘宗芳[1] 张丽华[1] 简立国[1] 汪涛[2] 

机构地区：[1]郑州大学第二附属医院心内科,河南郑州450014 [2]郑州大学第二附属医院干部病房,河南郑州450014

出　　处：《西安交通大学学报（医学版）》2016年第5期631-635,共5页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：河南省科技攻关计划项目(122102310088;132102310142;142102310109)~~

摘　　要：目的观察大鼠心肌梗死后心肌营养素-1(CT-1)的表达,并用骨髓间充质干细胞移植对其干预,探讨CT-1与心室重塑之间的关系。方法随机选取Wistar雄性大鼠50只,10只作为假手术组(A组);40只作为心肌梗死组,结扎左冠状动脉前降支(LAD)致心肌梗死24h后,分别随机抽取一半数量存活大鼠作为心肌梗死对照组(B组)及BMSC移植干预组(C组)。BMSC移植干预组(C组)通过尾静脉注射1mL BMSC细胞悬液于大鼠体内;A组、B组分别注射无血清培养液DMEM 1mL,6周后采用Real time PCR及免疫组化、Western blotting分别检测非梗死区CT-1的mRNA及蛋白表达量。结果 6周后,B组非梗死区心肌样本中,CT-1mRNA及蛋白表达量、左心质量指数,同A、C组相比均显著升高,且差异有统计学意义(P<0.05);C组非梗死区心肌中CT-1mRNA及其蛋白含量、左心室质量指数均较A组显著升高,差异具有统计学意义(P<0.05)。与B组相比,C组以上指标则明显下降,差异有统计学意义(P<0.05)。结论骨髓间充质干细胞能够抑制大鼠心肌梗死后CT-1mRNA及蛋白表达,从而改善心肌重构。Objective To investigate the effect of bone marrow mesenchymal stem cells（BMSCs）transplantation on the expression of cardiotrophin-1（CT-1）in ventricular remodeling after acute myocardial infarction in rats.Methods We randomly divided 50 male Wistar rats into sham operation group A（sham operation group,n=10）and acute myocardial infarction group（n=40）.Rats in acute myocardial infarction group were inflicted by ligating the left anterior descending coronary artery.After 24 hours,40 survived rats were randomly divided into group B（myocardial infarction control group,n=20）and group C（BMSC group,n=20）.Rats in group C were treated with BMSC transplantation,and those in group A and group B were treated with serum-free DMEM medium.After 6 weeks,the expressions of CT-1 at the mRNA and protein levels were detected by Real-time PCR method,immunohistochemical and Western blotting methods.Results After 6 weeks,the expressions of CT-1 at the mRNA and protein levels and left ventricular weight index in the myocardium of noninfraction zone in group B were significantly higher than those in group A and group C（P〈0.05）.The expressions of CT-1 mRNA and protein and left ventricular weight index in the myocardium of non-infraction zone in group C were significantly higher than those in group A（P〈0.05）.Conclusion BMSCs transplantation may improve ventricular remodeling after acute myocardial infraction in rats via inhibiting the expressions of CT-1 at the mRNA and protein levels.

关 键 词：骨髓间充质干细胞 心肌营养素-1 心肌梗死 心室重塑 

分 类 号：R542.2[医药卫生—心血管疾病]