Electrophoretic-deposited novel ternary silk fibroin/graphene oxide/hydroxyapatite nanocomposite coatings on titanium substrate for orthopedic applications  

作　　者：Ming LI Pan XIONG Maosong MO Yan CHENG Yufeng ZHENG 

机构地区：[1]Institute of Electrical Engineering, Chinese Academy of Sciences, Beijing 100190, China [2]School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China [3]Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China [4]Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China

出　　处：《Frontiers of Materials Science》2016年第3期270-280,共11页材料学前沿（英文版）

基　　金：This work was supported by the Beijing Natural Science Foundation （Grant No. 2164073）, the National Natural Science Foundation of China （Grant Nos. 21271032, 31370954 and 51431002）, the Scientific and Technical Program of Beijing （Grant No. Z141100002814008）, and the Innovative-Talent Program （Institute of Electrical Engineering, Chinese Academy of Sciences）.

摘　　要：The combination of graphene oxide （GO） with robust mechanical property, silk fibroin （SF） with fascinating biological effects and hydroxyapatite （HA） with superior osteogenic activity is a competitive approach to make novel coatings for orthopedic applications. Herein, the feasibility of depositing ternary SF/GO/HA nanocomposite coatings on Ti substrate was firstly verified by exploiting electrophoretic nanotechnology, with SF being used as both a charging additive and a dispersion agent. The surface morphology, microstructure and composition, in vitro hemocompatibility and in vitro cytocompatibility of the resulting coatings were investigated by SEM, Raman, FTIR spectra and biocompatibiUty tests. Results demonstrated that GO, HA and SF could be co-deposited with a uniform, smooth thin-film morphology. The hemolysis rate analysis and the platelet adhesion test indicated good blood compatibility of the coatings. The human osteosarcoma MG63 cells displayed well adhesion and proliferation behaviors on the prepared coatings, with enhanced ALP activities. The present study suggested that SFIGO/HA nanocomposite coatings could be a promising candidate for the surface functionalization of biomaterials, especially as orthopedic implant coating.The combination of graphene oxide （GO） with robust mechanical property, silk fibroin （SF） with fascinating biological effects and hydroxyapatite （HA） with superior osteogenic activity is a competitive approach to make novel coatings for orthopedic applications. Herein, the feasibility of depositing ternary SF/GO/HA nanocomposite coatings on Ti substrate was firstly verified by exploiting electrophoretic nanotechnology, with SF being used as both a charging additive and a dispersion agent. The surface morphology, microstructure and composition, in vitro hemocompatibility and in vitro cytocompatibility of the resulting coatings were investigated by SEM, Raman, FTIR spectra and biocompatibiUty tests. Results demonstrated that GO, HA and SF could be co-deposited with a uniform, smooth thin-film morphology. The hemolysis rate analysis and the platelet adhesion test indicated good blood compatibility of the coatings. The human osteosarcoma MG63 cells displayed well adhesion and proliferation behaviors on the prepared coatings, with enhanced ALP activities. The present study suggested that SFIGO/HA nanocomposite coatings could be a promising candidate for the surface functionalization of biomaterials, especially as orthopedic implant coating.

关 键 词：graphene oxide silk fibroin HYDROXYAPATITE nano-biocomposite coatings ELECTRODEPOSITION 

分 类 号：O613.71[理学—无机化学] TQ325.12[理学—化学]