低盐驯化对半滑舌鳎幼鱼生长、渗透生理及糖代谢酶活力影响的研究  

The Growth Performance,Osmotic Physiology and Carbohydrate Metabolism Activity of Juvenile Cynoglossus semilaevis Acclimated to Low Salinity Water

作　　者：房子恒[1] 田相利[1] 董双林[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2016年第8期19-27,共9页Periodical of Ocean University of China

基　　金：国家"十二五"科技支撑计划项目(2011BAD13B03);山东省农业重大应用技术新项目(鲁财农指[2013]136号)资助~~

摘　　要：为研究半滑舌鳎（Cynoglossus semilaevis）幼鱼在不同盐度条件下的适应性,将半滑舌鳎幼鱼在淡水（S0）和盐度5（S5）、盐度10（S10）、盐度20（S20）、盐度30（S30）条件下驯养60d,测量半滑舌鳎幼鱼的生长、渗透生理和糖代谢酶活力等指标。实验表明：盐度降低至10-20时,对半滑舌鳎幼鱼的生长具有一定的促进作用,在S10时鳃和肾脏中的Na＋/K＋-ATPase活力最低（P〈0.05）。通过检测鱼体的糖代谢酶活力发现：鳃中丙酮酸激酶（PK）活力在S10附近有最小值,琥珀酸脱氢酶（SDH）和苹果酸脱氢酶（MDH）活力在S10、S5及S0时均显著降低（P〈0.05）,表明鳃中柠檬酸循作用在驯化至低盐度后有所减弱,在淡水条件下糖酵解作用有所增强;在淡水条件下肾脏中己糖激酶（HK）、PK的活力最高（P〈0.05）,SDH和MDH活力较高,表明淡水中幼鱼肾脏中糖酵解和三羧酸循环作用有所增强;在淡水条件下肝脏中HK和SDH活力有所增强,表明淡水中幼鱼肝脏中的糖代谢作用有一定程度的增强。研究结果表明,淡水条件下,半滑舌鳎幼鱼的代谢水平始终维持在较高状态,表现为渗透压调节器官中糖代谢作用有所加强,生长受到抑制,在S10附近鱼体代谢率低,代谢酶活力处在较低水平,有利于幼鱼生长。To evaluate the capability of juvenile tongue sole adapted to different salinity conditions, the growth, osmotic physiology and carbohydrate metabolic enzyme activity of juvenile tongue sole acclima- ted to fresh water （SO）, S5, S10, S20 and S30 for 60 days were determined. The results showed that the growth increased at S10 - S20, and the lowest Na＋/K＋-ATPase activity in gills and kidney was found at S10 （P〈0.05）. Under the freshwater condition, the plasma osmolality of juvenile tongue sole was significantly lower compared with the normal level, while the Na＋/K＋-ATPase activity in the kid- ney was greatly improved, and the growth performance of juvenile tongue sole decreased significantly. Food conversion efficiency of tongue sole were found decreased at SO and S30, which were significantly lower than that of other treatments （P〈0. 05）. The minimum apparent digestive rate was obtained at S30, which was significantly lower than that at S5, S10 and S20 （P〈0. 05）. The carbohydrate metabol- ic enzymes were also detected in different tissues of tongue sole. The minimum value of pyruvate kinase （PK） activity in gills was found at S10, while a maximum value was found at S0 and a higher level of the activity was found at S30. The activity of succinate dehydrogenase （SDH） and malate dehydrogenase （MDH） at S10, S5 and SO were significantly decreased （P〈0. 05） in gills of tongue sole. All these showed that glycolysis and the citric acid cycle in gills reduced when acclimated to lower salinity, whereas glycolysis increased at SO. In kidney hexokinase （HK）, PK activity reached the highest level at SO, which was significant higher than the other treatments （P〈0. 05） higher activity of SDH and MDH were also found at SO, which demonstrated that glycolytic and tricarboxylic acid cycle all enhanced in kindey of tongue sole in fresh water condition. In brief, when acclimated to the freshwater, plasma os- motic of tongue sole could not be recorvered to normal levels, the fis

关 键 词：半滑舌鳎 盐度 生长 渗透生理 糖代谢 

分 类 号：S917.4[农业科学—水产科学]