发光二极管光源照射日本大耳白兔皮肤的安全性评价  

The safety assessment on the light emitting diode(LED) resource through irradiating the skin of Japanese big-ear white rabbits

作　　者：李彦红[1] 张继刚[2] 徐艳峰[1] 韩云林[1] 黄澜[1] 江彬彬[2] 朱华[1] 徐玉环[1] 杨维玲[2] 秦川[1] 

机构地区：[1]中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,新发再发传染病动物模型研究北京市重点实验室,北京100021 [2]中国人民解放军火箭军总医院,北京100088

出　　处：《中国比较医学杂志》2016年第7期24-30,共7页Chinese Journal of Comparative Medicine

基　　金：十二五重大科技专项(2012ZX10004-501);国家高技术研究发展计划(863计划)课题子任务(2003AA123310)

摘　　要：目的通过LED白色光源照射日本大耳白兔皮肤组织,对LED光源照射的安全性进行初步的评价。方法动物随机分成照射组和对照组。照射组动物剔除背部毛发,LED光源照射,强度为50 mw/cm2,对照组动物以普通日光灯光源照射,两组均每天照射5 h,连续3个月。观察动物皮肤有无红肿,测量动物进食、体重、体温等改变情况;照射完毕采集外周血测量常规、生化指标;Elisa分析免疫细胞及细胞因子改变情况;脏器组织病理检测,及分析皮肤弹力纤维变化情况;皮肤免疫组织化学分析C-myc、P53、细胞周期蛋白D1(CCND1)等癌基因及抑癌基因的表达变化。结果照射组兔皮肤未见红肿表现,体重在6周后稍高于对照组,体温3周、6周有差别,进食未见异常;血常规有白细胞增多,生化检测尿素、肌酐水平有增高,但在正常范围内;ELISA分析血免疫细胞CD3T细胞、细胞因子IL-6、TNF-α、IFN-γ水平在两组无明显差别,照射组动物CD19B细胞,IL-4水平升高;照射组动物脏器组织病理未见明显异常结构改变,皮肤弹性纤维分布无异常;皮肤组织内细胞周期蛋白(CCND1),C-Myc蛋白,P53蛋白表达与对照组相比没有差异。结论 LED光源照射日本大耳白兔3个月后,未引起体内明显的组织病理学改变,也未引起皮肤肿瘤相关因子的改变。Objective To evaluate the safety of Light emitting diode （LED） source through irradiating the skin of Japanese big-ear white rabbits. Methods Animals were randomly divided into irradiated group and control group. Animals in the two groups were irradiated with LED light source after eliminating back hair （50mw/cm2） and the ordinary fluorescent lamp, respectively. The animals were irradiated for 5 hours every day for 3 months. The changes of animal skin symptoms, feeding, body weight, and temperature were observed and detected; the blood routine and biochemical indexes were tested; the changes of haematological immune cells and eytokines were analyzed by ELISA ; histopathology and elastic fibers of skin were detected; the levels of C-myc, 1）53, and cyclin D1 in skin were tested by immunohistochemistry. Results The skin of rabbits in irradiated group showed no abnormal change ; the weight was higher than the control group animals at the 6th week, and the temperature had difference at the 3rd and 6th week, the diet showed no abnormal. Blood routine showed white blood cells increased; biochemical detection manifested urea and creatinine levels increased, but the indexes are in normal range; the hematological CD3T cells, cytokines IL-6,TNF-a and IFN-Y/in the two group animals had no significant difference. The levels of CD19B cell and IL-4 level increased in irradiated group; The viscera histopathology structure and skin elastic fibers distribution in irradiated group animals showed normal; the expression of cell cycle protein 1 （ CCND1 ）, c-mye protein and P53 protein in skin of irradiated group had no difference with control. Conclusions After irradiating 3 months, the LED light source had not caused the obvious pathology damage of tissues and change of skin tumor related factors to the rabbits.

关 键 词：发光二极管 日本大耳白兔 皮肤 安全评价 

分 类 号：R332[医药卫生—人体生理学]