黄河鲤全同胞家系的微卫星标记亲子鉴定  

Parentage assignment for full-sib families of the Yellow River carp Cyprinus carpio haematoperus based on microsatellites

作　　者：王新华[1,2] 俞小牧[1] 冯建新 童金苟[1] 

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,湖北武汉430072 [2]中国科学院大学,北京100039 [3]河南省水产科学研究院,河南郑州450044

出　　处：《中国水产科学》2016年第5期1023-1031,共9页Journal of Fishery Sciences of China

基　　金：公益性行业(农业)科研专项项目(200903045);淡水生态与生物技术国家重点实验室课题(2011FBZ20)

摘　　要：本研究利用11个高度多态的微卫星标记对26个黄河鲤(Cyprinus carpio haematoperus)全同胞家系中的417尾体重较大的正常体色个体(黄河鲤较大个体)和52尾红色个体(红鲤表型个体)进行了亲子鉴定。11个微卫星座位的平均等位基因数、平均观测杂合度(H_o)、平均期望杂合度(H_e)和平均多态信息含量(PIC)分别为8.2、0.792、0.792和0.76。当双亲未知且置信度为95%时,11个座位的累积排除概率达99.79%。除29尾黄河鲤较大个体以外,388尾黄河鲤较大个体和52尾红鲤表型个体准确地找到了其父母本,实际鉴定率为93.82%。通过对子代数目超过20尾的7个黄河鲤家系生长性状的比较分析,本研究成功鉴定出子代生长性状优良的黄河鲤亲本组合及其家系,同时也鉴定出包含隐性红色基因的黄河鲤杂合亲本,为进一步选育表型和体色纯正且生长快速的黄河鲤新品种提供了理论依据和技术手段。Parentage assignment is a powerful tool for pedigree analysis and family selection in aquatic animals. Eleven polymorphic microsatellite markers were used to perform a parentage assignment for 417 fast-growth progeny with normal color and 52 red progeny from 26 full-sib families of Yellow River carp, Cyprinus carpio haematoperus. The average number of alleles for the 11 microsatellites was 8.2, and the mean values of observed heterozygosity, expected heterozygosity, and polymorphism information content were 0.792, 0.792, and 0.76, respectively. The combined probability of exclusion reached 99.79% when both parents were unknown （confidence level, 95%）. Among the carp progeny, 29 were not assigned, but 388 fast-growth progeny and 52 red progeny were accurately assigned parental pairs, resulting in an identification rate of 93.82%. By comparison of growth performance among seven families with 20 or more progeny, we identified several favorable parents and families that should be kept for further selection. For body color, two parents with recessive alleles for the red color were identified. These results provide the basis and molecular tools for further selection towards breeding new varieties of fast-growing Yellow River carp with the typical slim body shape and golden color.

关 键 词：黄河鲤 亲子鉴定 微卫星 鉴定率 

分 类 号：S917[农业科学—水产科学]