无菌猪生长、血液以及脏器相关指标的测定  

Measurement of body weight,blood parameters and main organ coefficients of germ-free piglets

作　　者：孙静[1,2,3] 杜蕾[4] 丁玉春[1,2,3] 曹浩然[1,2,3] 吴梦[1,2,3] 林保忠[1,2,3] 张世华[1] 刘作华[1,2,3] 葛良鹏[1,2,3] 

机构地区：[1]重庆市畜牧科学院,重庆402460 [2]农业部养猪科学重点实验室,重庆402460 [3]养猪科学重庆市市级重点实验室,重庆402460 [4]西南大学荣昌校区,重庆荣昌402460

出　　处：《中国实验动物学报》2016年第4期388-394,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家"863"计划(2014AA021602);重庆市国际合作项目(CSTC2013gjhz80002);重庆市基础与前沿研究(cstc2013jcyj C80001);重庆市农发资金(12402)资助项目;无菌动物应用示范平台(cstc2015pt-kjyfsf0024)

摘　　要：目的建立无菌太湖猪多项生理数据背景资料。方法对4头25 d无菌猪和4头同窝普通级群体,雌雄比3∶1,测定并比较血液常规、生化、免疫球蛋白和主要脏器发育指标。运用异速生长建模,对主要脏器重量相对体重的异速生长进行了分析。结果 (1)无菌猪与普通级猪的12项血常规和8项血生化指标差异有显著性(P<0.05);(2)无菌猪与普通级猪的平均体重、血清中Ig M表达量差异达到显著性水平(P<0.05),且无菌猪缺少肠系膜淋巴结;(3)无菌猪的心脏、脾和胃与体重呈负异速生长(异速生长指数b<1),肠道与体重的异速生长指数为1.78,为正异速生长;而普通级猪的心脏、胃和肠道与体重呈负异速生长(b<1),肾与体重的异速生长指数最大,为1.20,呈正异速生长。结论微生物学等级差异影响了猪体重、血液及脏器、免疫系统的发育。Objective To establish the background information of physiological parameters for germ-free（ GF）Taihu piglets. Methods In this study we selected 25 days old GF Taihu piglets and 4 conventional（ CV） littermates,the male and female ratio was 1∶ 3,to measure the normal clinical values of hematology and serum biochemistry,immunoglobulin concentration and main organ coefficients. The analysis of relative growths of main organ weight to body weight was conducted in the Taihu GF and CV pigs by allometric scaling model. Results（ 1） Twelve hematological parameters and 8blood biochemical parameters in the GF piglets were significantly lower than those in CV pigs（ P 〈 0. 05）.（ 2） The average body weight,IgM concentration of GF pigs and CV pigs had significant difference（ P 〈 0. 05）,and no mesenteric lymph nodes were found in the GF pigs.（ 3） The gut weight had the largest allometric association with body weight in the GF pigs,while spleen weight has the largest allometric association with body weight in the CV pigs. Both the weight of heart and stomach in CV and GF pigs had a negative allometric association with body weight（ allometric coefficient b 〈 1）,respectively. Conclusions Different microbe control grades affect the body weight,hematology and serum biochemistry,expression of immunoglobulin and development of main organs in laboratory pigs.

关 键 词：无菌猪 血液参数 脏器发育 异速生长分析 

分 类 号：Q95-33[生物学—动物学]