稳定的大鼠大脑中动脉栓塞脑梗死模型的建立  

Establishment of a stable rat model of middle cerebral artery occlusion

作　　者：包新杰[1] 李雪元[1] 左赋兴[1] 冯铭[1] 窦万臣[1] 王任直[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院神经外科,北京100730

出　　处：《中国实验动物学报》2016年第4期395-398,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(81200916);国家高技术研究发展计划(863计划)(2014AA020513;2013AA020106)

摘　　要：目的探讨使用激光多普勒血流监测技术制作稳定的大鼠大脑中动脉栓塞脑梗死模型（middle cerebral artery occlusion,MCAO）的可行性。方法 16只SD雄性大鼠随机分成2组：实验组和对照组各8只。实验组将模型制作过程中脑血流下降至基础值的30%判定为模型制作成功;对照组不监测脑血流,将尼龙栓线插入深度为1.8 cm判定为模型制作成功。模型前和模型后24 h分别行神经损伤严重程度评分（modified neurological severity scores,m NSS）;模型后24 h处死大鼠取脑,行2,3,5-三苯氯化四氮唑（TTC）染色并计算脑梗死体积。结果实验组8只大鼠模型后24 h均出现典型偏瘫症状,m NSS评分稳定在10分~13分,梗死体积稳定性和均一性好,为（37.5±3.9）%。对照组8只大鼠m NSS评分稳定性较差,其中5只大鼠的m NSS评分为10分~13分,5只大鼠的脑梗死灶和实验组相似,但有3只大鼠的脑梗死体积明显小于实验组（P〈0.05）。实验组的模型成功率为100%,对照组的模型成功率为62.5%（P〈0.05）。结论激光多普勒血流监测技术可以显著提高大鼠MCAO模型的成功率、稳定性和均一性。Objective To establish a stable rat model of middle cerebral artery occlusion（ MCAO） assisted by laser Doppler flowmetry. Methods Sixteen rats were randomly divided into two groups：（ 1） experimental group,n = 8;（ 2） control group,n = 8. In the experimental group,a sudden decrease of regional cerebral blood flow from baseline（ before ischemia） to approximately 30% of baseline confirmed the correct placement of nylon thread. In the control group,1. 8cm nylon thread insertion was performed without laser Doppler flowmetry. The modified neurological severity scores（ m NSS） were tested before and 24 h after MCAO. All rats were killed at 24 h after MCAO. The brains were removed and dissected into 6 pieces of 2 mm coronal sections. The fresh brain slices were stained with 2% 2,3,5-triphenyl tetrazolium chloride and the relative infarct volume was measured. Results In the experimental group,8 rats showed typical symptom of hemiplegy with stable m NSS（ between 10 and 13） and infarct volume（ 37. 5 ± 3. 9%）. However,only 5 rats in the control group showed stable m NSS and infarct volume. The success rate of the model establishment was 100% in the experimental group compared with 62. 5% in the control group（ P 〈 0. 05）. Conclusion The application of laser Doppler flowmetry significantly increases the establishment success rate of stable MCAO models in rats.

关 键 词：脑梗死 模型 激光多普勒 中动脉 栓塞 大鼠 

分 类 号：Q95-33[生物学—动物学]