肠复康方对急性放射性肠炎大鼠小肠黏膜细胞凋亡基因的影响  

Effect of Changfukang Prescription on Apoptotic Genes in Small Intestinal Mucosa in Rats with Acute Radiation Enteritis

作　　者：李思聪[1] 李玉明 

机构地区：[1]湖南中医药大学,湖南长沙410007 [2]湖南省肿瘤医院中南大学湘雅医学院附属肿瘤医院,湖南长沙410013

出　　处：《安徽中医药大学学报》2016年第4期67-73,共7页Journal of Anhui University of Chinese Medicine

基　　金：湖南省中医药管理局一般课题(201177)

摘　　要：目的探讨肠复康方对急性放射性肠炎大鼠一般情况及小肠黏膜相关细胞凋亡基因的影响。方法以X线直线加速器给予实验大鼠全腹照射,建立急性放射性肠炎大鼠模型。将实验大鼠随机分成正常对照组,模型对照组,肠复康方低、中、高剂量组。模型复制成功后,肠复康各剂量组给予10mL/kg灌胃,低、中、高剂量组剂量换算后分别含生药15.75、31.50、63.00g/kg,模型对照组、正常对照组同时予等量生理盐水,连续30d灌胃。期间观察大鼠精神状态、摄食进水、排便情况及体质量变化,处死后取相应部位的小肠用免疫组化及Western blot方法检测黏膜上皮细胞中凋亡基因Caspase-3、Bax、Bcl-2的表达。结果肠复康方干预大鼠的精神状态、饮食进水、排便情况较模型对照组改善;与模型对照组比较,肠复康方干预大鼠体质量显著增加,以中剂量组效优(P<0.05)。肠复康方低、中、高剂量组小肠黏膜的凋亡基因Caspase-3、Bax和Bax/Bcl-2表达较模型对照组显著下调、Bcl-2显著上调(P<0.05),中剂量效优。结论肠复康方具有改善急性放射性肠炎大鼠一般情况及抑制其细胞凋亡作用,以中剂量效优,其机制可能与下调小肠黏膜上皮凋亡基因Caspase-3、Bax表达及上调Bcl-2的表达,抑制细胞凋亡有关。Objective To investigate the effect of Changfukang prescription on general condition and apoptotic genes in the small intestinal mucosa in rats with acute radiation enteritis.Methods An X-ray linear accelerator was used to perform total abdominal irradiation for experimental rats to establish the rat model of acute radiation enteritis.The experimental rats were randomly divided into normal control group,model control group,and low-,medium-,and high-dose Changfukang groups.After the model was successfully established,all rats in the low-,medium-,and high-dose Changfukang groups were given 10 mL/kg Changfukang decoction by gavage,equivalent to doses of the crude drug of 15.75g/kg,31.50g/kg,and63.00g/kg,respectively,as was determined by dose conversion.The rats in the model control group and the normal control group were given normal saline of the same volume by gavage.All the rats were treated by gavage for 30 days continuously.The mental state,food and water intake,defecation,and change in body weight were observed.After the rats were sacrificed,the corresponding small intestinal tissues were harvested,and immunohistochemistry and Western blot were used to measure the expression of apoptotic genes Caspase-3,Bax,and Bcl-2in small intestinal epithelial cells.Results Compared with the model control group,the Changfukang groups showed significant improvements in the mental state,food and water intake,and defecation,as well as significant increases in body weight,and the medium-dose Changfukang group showed the best response（P〈0.05）.Compared with the model control group,the low-,medium-,and high-dose Changfukang groups showed significantly downregulated expression of Caspase-3and Bax,a significant decrease in Bax/Bcl-2ratio,and significantly upregulated expression of Bcl-2（P〈0.05）,and the medium-dose Changfukang group showed the best response.Conclusion Changfukang prescription can improve the general condition of rats with acute radiation enteritis and inhibit cell apoptosis,and the medium-dose Changfukang

关 键 词：肠复康方 放射性肠炎 动物模型 细胞凋亡 

分 类 号：R516.1[医药卫生—内科学]