干旱锻炼对盐胁迫下水稻幼苗根系抗氧化酶活性的影响  

Effect of PEG pretreatment on antioxidant enzymes activity under salt stress in root of rice seedling

作　　者：梅映学 魏玮[1] 张诗婉 张韫璐 王金缘 王茜[1] 苏昕[1] 马莲菊[1] 

机构地区：[1]沈阳师范大学生命科学学院,辽宁沈阳110034

出　　处：《浙江农业学报》2016年第8期1304-1308,共5页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金资助项目(31270369);沈阳师范大学生态与环境研究中心主任基金资助项目(EERC-K-201406);沈阳师范大学"大学生创新创业训练计划"项目;北方粳稻遗传育种省部共建教育部重点实验室/农业部东北水稻生物学与遗传育种重点实验室(沈阳农业大学)开放课题

摘　　要：采用PEG预处理对辽星1号水稻幼苗进行干旱锻炼,研究其对盐胁迫下水稻幼苗根系的缓解作用。水稻幼苗培养1周后用10%PEG-6000预处理3 d,然后复水3 d,最后用100 mmol·L-1Na Cl胁迫处理3 d。测定水稻幼苗根系的相对含水量、根系活力、丙二醛含量及抗氧化酶(SOD、POD、CAT、APX)活性。结果表明,PEG预处理的水稻幼苗根系MDA含量显著低于盐胁迫的处理(P<0.05),而幼苗根系相对含水量、根系活力以及抗氧化酶活性则均高于盐胁迫处理。说明PEG预处理可以提高水稻幼苗根系的耐盐性,缓解水稻幼苗在盐胁迫下所受的部分伤害。Effects of PEG pretreatment on rice Liaoxing 1 seedling root antioxidative activities stressed by salt stress were studied. The experiment materials were dealt with 10% PEG-6000 pretreatment for 3 d,next rewatered for 3 d, and then stressed for 3 d with 100 mmol·L^-1 NaCl. Water content,root activity,MDA content and antioxidant enzymes （SOD,POD,CAT, APX） activity in rice seedling roots were deter^nined. The results showed that MDA content of PEG pretreatment in roots of rice seedling was significantly lower than that of salt stressed （ P 〈 0. 0 5 ） ; However, water content, root activity and activities of antioxidant enzymes with PEG pretreatment were higher than those of salt stressed. This showed that PEG pretreatment could improve the resistance of rice seedlings and alleviate the damage of salt stress on rice seedlings.

关 键 词：PEG预处理 盐胁迫 水稻 抗氧化酶活性 

分 类 号：S511[农业科学—作物学]