牦牛CAPN1基因intron 1多态性及其与胴体和肉质性状的关联性  

Polymorphism in the intron 1 of CAPN1 and its association with carcass and meat quality traits in yak

作　　者：陈杰[1] 田知利 胡江[1] 罗玉柱[1] 刘秀[1] 王继卿[1] 石红梅 雷俊杰 

机构地区：[1]甘肃农业大学动物科学技术学院/甘肃省草食动物生物技术重点实验室,甘肃兰州730070 [2]甘南州畜牧科学研究所,甘肃合作747000

出　　处：《浙江农业学报》2016年第8期1315-1322,共8页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金项目(31160451);甘肃省杰出青年科学基金项目(1210RJDA008);现代农业(肉牛牦牛)产业技术体系建设专项(CARS-38)

摘　　要：采用PCR-SSCP技术检测甘南牦牛、天祝白牦牛及大通牦牛钙蛋白酶1(CAPN1)基因单核苷酸多态性(SNPs),并分析SNPs对甘南牦牛部分胴体及肉质性状的影响。结果表明,3类群牦牛在CAPN1基因第1内含子区检测到g.100+606G>T突变,形成了AA、BB和AB 3种基因型,检测区域表现为中度多态;CAPN1基因第1内含子突变影响不同年龄段甘南牦牛肌肉嫩度、失水率和眼肌面积,AA基因型个体嫩度剪切力值较低而眼肌面积较高,其中3岁和5岁牦牛AA型个体嫩度剪切力显著低于BB或AB型(P<0.05),4岁和5岁牦牛AA型个体眼肌面积显著高于BB或AB型(P<0.05);另外6岁牦牛AA型个体失水率显著高于BB和AB型(P<0.05)。CAPN1基因g.100+606G>T突变可用为甘南牦牛此类性状潜在的遗传标记之一。The single nucleotide polymorphisms （ SNPs） of CAPA1 gene in Gannan yak,Tianzhu yak and Datong yak were detected by the technology of PCR-SSCP. The relationship between SNPs and Gannan yak carcass and meat quality traits was analyzed. The results of PCR-SSCP indicated that there was a genetic mutation of g. 100 ＋606G 〉 Tin the first introns of CAPA1 gene of all 3 groups to form 3 genotypes （ AA,AB and BB）. Genetic analyses of CAPA1 gene showed that the polymorphism information contents in the detection region were 0. 25 〈 PIC 〈 0. 50, which belonged to a moderate polymorphism. Meat tenderness degree,water loss rate,eye muscle area of different age periods of Gannan yaks were regulated by genetic mutation of CAPA1. Lower shear force was observed in AA type yaks aged 3 and 5 years when compared with BB and AB type yaks （ P 〈 0. 05 ） . Higher eye muscle area was ob-served AA type yaks aged 4 and 5 years old when compared with BB and AB type yaks （ P 〈 0. 05）. Moreover, highei,water loss rate was observedin 6 years old AA type yaks when compared with BB and AB type yaks （ P 〈 0. 05）. Genetic mutation of g. 100 ＋606G 〉T could be used as genetic markers.

关 键 词：甘南牦牛 CAPN1基因 多态性 胴体和肉质性状 

分 类 号：S823.85[农业科学—畜牧学]