Effects of H_2O_2 and NO on Flower Development and AP1 Gene Expression of Off-season Longan  

作　　者：Songgang LI Xinjun ZHANG Lei ZHANG Jiwang HONG Yeyuan CHEN 

机构地区：[1]Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture/National Cultivar Improvement Center of Tropical Fruit Tree,Danzhou 571737,China [2]Henan Vocational College of Agriculture,Zhengzhou 451400,China

出　　处：《Agricultural Biotechnology》2016年第4期23-25,30,共4页农业生物技术（英文版）

基　　金：Supported by China Agriculture Research System(CARS-33-25)

摘　　要：[Objective] This study aimed to investigate the effects of H2O2 and NO on flower development and 4P1 gene expression of off-season longan. [Meth-od ]Nine-year-old off-season Chuliang longan was sprayed with enhancers and blockers of H2O2 and NO to analyze dynamic changes of flower development and API expression. [ Result] The expression level of API gene in off-season longan was improved during flower development. SNP and MV treatments up-regulated the expression level of API gene in leaves and terminal buds to varying degrees during lateral primordium formation. Especially, SNP treatment exhibited the most remark-able effect. DMTU treatment significantly inhibited the expression of AP1 gene in leaves and terminal buds during lateral primordium formation. L-NNA treatment slightly inhibited the expression of API gene in leaves and exerted no significant effect on expression of AP1 gene in terminal buds. [ Conclusion] It can be specu-lated that the enhancement of NO and H2O2 signals is conducive to flower development of off-season longan, while blocking H2O2 signals may inhibit flower develop-ment of off-season longan.[Objective] This study aimed to investigate the effects of H2O2 and NO on flower development and 4P1 gene expression of off-season longan. [Meth-od ]Nine-year-old off-season Chuliang longan was sprayed with enhancers and blockers of H2O2 and NO to analyze dynamic changes of flower development and API expression. [ Result] The expression level of API gene in off-season longan was improved during flower development. SNP and MV treatments up-regulated the expression level of API gene in leaves and terminal buds to varying degrees during lateral primordium formation. Especially, SNP treatment exhibited the most remark-able effect. DMTU treatment significantly inhibited the expression of AP1 gene in leaves and terminal buds during lateral primordium formation. L-NNA treatment slightly inhibited the expression of API gene in leaves and exerted no significant effect on expression of AP1 gene in terminal buds. [ Conclusion] It can be specu-lated that the enhancement of NO and H2O2 signals is conducive to flower development of off-season longan, while blocking H2O2 signals may inhibit flower develop-ment of off-season longan.

关 键 词：LONGAN Flower development Hydrogen peroxide （H2O2） Nitric oxide （NO） API gene 

分 类 号：S667.2[农业科学—果树学]