霜霉病高效生防菌株BCJB01发酵培养基的优化  

Optimization of fermentative medium for highly effective biocontrol strain Bacillus cereus BCJB01 against downy mildew

作　　者：王贻莲[1] 谢雪迎[1] 扈进冬[1] 陈凯[1] 杨合同[1] 李纪顺[1] 

机构地区：[1]山东省科学院生物研究所,山东省应用微生物重点实验室,山东济南250014

出　　处：《山东科学》2016年第4期30-34,共5页Shandong Science

基　　金：山东省重点研发计划(2015GNC113002);山东省科学院-枣庄市产业技术研发联合基金(201503);公益性行业(农业)科研专项(201203035)

摘　　要：为提高霜霉病高效生防菌株Bacillus cereus BCJB01的发酵水平,通过正交试验、综合优选及糖分流加试验,优化BCJB01的发酵培养基。优化出的培养基配方的质量分数为：玉米粉2.00%、豆粕粉1.00%、四水氯化锰0.003 0%、十二水磷酸氢二钠0.30%、二水磷酸二氢钠0.15%、七水硫酸镁0.035%、氯化钙0.10%和酵母提取物0.30%,接菌后6-12 h流加质量分数0.2%糖。本研究优化的培养基配方在接种量为3%（V/V）,转速为180 r/min,温度为30℃的摇床振荡培养至66 h时,芽孢率近90%,菌体数量达100×10^8cfu/m L以上。To improve fermentation level of Bacillus cereus BCJBOI, a highly efficient biocontrol strain against downy mildew, we optimized its fermentative medium through orthogonal test, comprehensive optimization and sugar feeding tests. Mass fraction of the optimized medium formula are 2. 00% corn meal, 1.00% soybean powder, 0. 0030% manganese chloride tetrahydrate, 0. 30% twelve aqueous disodium hydrogen phosphate, 0. 15% sodium dihydrogen phosphate dihydrate, 0.035% magnesium sulfate heptahydrate, 0.10% calcium chloride, 0. 30% yeast extract, 0. 2%sugar added at 6 -12 h after inoculation. It can produce more than 100× 10^8 cfu/mL cells and its spore rate is 90%,when the cultivation conditions are 3% （ V/V） inoculum size, 180r/ min rotation velocity, 30 0C shaky cultivation till 66 hours.

关 键 词：蜡样芽孢杆菌 霜霉病 发酵培养基 正交设计 综合优选 

分 类 号：Q939.95[生物学—微生物学]