猪胚胎中差异表达基因检测方法的研究（英文）  

Study on the detection methods of differentially expressed genes in porcine embryos

作　　者：马天泽[1] 李华[2] 林昌岫[2] 

机构地区：[1]延边大学附属医院血液科 [2]延边大学附属医院中心实验室,吉林延吉133000

出　　处：《延边大学医学学报》2016年第2期81-84,共4页Journal of Medical Science Yanbian University

基　　金：延边大学科研项目(2013300-601010007);吉林省卫生计生委课题(2014ZC056);吉林省教育厅"十二五"科学技术研究项目(吉教科合字[2015]第45号)

摘　　要：[目的]探讨不同检测方法检测猪胚胎中差异表达基因的效果.[方法]采用现行的ACPs differential display技术和改良的ACP方法检测人工授精和核移植猪胚胎中的差异表达基因.改良的ACP方法中第2次cDNA合成过程和DNA扩增过程在分开的步骤中执行.[结果]采用现行的ACP方法无法获得真实扩增的DNA克隆,采用改良的ACP方法时获得了所愿的DNA克隆.[结论]在检测猪胚胎差异表达基因中改良的ACP方法较现行的ACP方法更为有效.OBJECTIVE To explore the effects of different kinds of methods on detecting differentially expressed genes （DEGs） in porcine embryos. METHODS Current annealing control primers （ACPs） differential display technique and modified ACP method were used to detect DEGs between in vitro fertilization （IVF） and nuclear transfer （NT）-derived porcine embryos. Second-strand cDNA synthesis and DNA exponential amplification were performed in the separated steps in modified ACP method. RESULTS Real amplified DNA clones could not be obtained by current ACP method, while modified ACP method could. CONCLUSION The modified ACP method was more effective than current ACP method in detecting DEGs in porcine embryos.

关 键 词：差异表达基因 猪胚胎 核移植 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]