不同基础培养基对漏斗形马勃菌丝体生长的影响  

Effects of Different Basic Media on the Mycelium Growth of Lycoperdon excipuliforme

作　　者：王君[1] 田强[1] 游朗 李玉延 宋力雅 马林[1,2] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621010 [2]四川省生物质资源利用与改性工程技术研究中心,四川绵阳621010

出　　处：《安徽农业科学》2016年第19期114-116,共3页Journal of Anhui Agricultural Sciences

基　　金：国家大学生创新创业训练计划(201410619002);西南科技大学大学生创新基金(CX15-040);西南科技大学生命科学与工程学院大学生创新实践项目(2014011)

摘　　要：[目的]研究漏斗形马勃在7种不同基础培养基上的生长情况。[方法]采用7种基础培养基进行试验,通过液体和固体培养,以菌丝体生物量及菌落直径为指标,确定漏斗形马勃的最佳基础培养基。[结果]最适合漏斗形马勃生长的基础培养基配方为玉米渣4%、蛋白胨0.3%、蔗糖2%、KH2PO41%、Mg SO40.05%。在28℃和摇床转速150 r/min条件下,液体培养5 d后的菌丝体干重为16.56 g/L。在28℃固体培养7 d,菌丝体鲜重和干重分别为16.47和2.27 g/L,菌落直径为4.49 cm。[结论]该研究为漏斗形马勃培养条件的进一步优化奠定了基础。[Objective]To study the mycelium growth of Lycoperdon excipuliforme in seven basic media. [Method]According to medium biomass and colony diameter as growth parameters,the optimal basic medium for the mycelium growth of L. excipuliforme was selected from seven liquid and solid media with different constituents. [Result] The optimal basic medium for the mycelium growth of L. excipuliforme was the IV medium composed of maize（ 4%）,peptone（ 0. 3%）,sucrose（ 2%）,KH2PO4（ 1%） and Mg SO4（ 0. 05%）. After the mycelia were cultured in the liquid medium at the rotational speed of 150 r / min at 28 ℃ for 5 d,their dry weight in the IV medium was 16. 56 g / L. When the mycelia were cultured in solid medium at 28 ℃ for 7 d,their fresh weight and dry weight in the IV medium were 16. 47 and 2. 27 g / L respectively,and the colony diameter was 4. 49 cm. [Conclusion] The study lays the foundation for the further optimization of culture conditions of L. excipuliforme.

关 键 词：漏斗形马勃 固体培养 液体培养 培养基 菌丝体 生长 

分 类 号：S567.3[农业科学—中草药栽培]