刺三甲对佐剂性关节炎大鼠踝关节滑膜组织NF-κB蛋白表达的影响  

Effect of Acanthopanax on the protein expression of NF-κB in ankle synovial tissue of AA rat

作　　者：童妍[1] 郝安辉 李坤伦[1] 金钊[2] 

机构地区：[1]西南交通大学生命科学与工程学院,成都610031 [2]成都中医药大学,成都610075

出　　处：《中药药理与临床》2016年第3期109-112,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：中央高校基本科研业务费专项资金项目(2682014CX047)

摘　　要：目的:观察刺三甲乙醇浸膏对佐剂性关节炎(AA)大鼠踝关节滑膜细胞核转录因子(NF-κB)表达的影响。方法:将60只SD大鼠随机分成6组:空白组,模型组,尼美舒利组,刺三甲高、中、低剂量组,采用大鼠佐剂性关节炎模型,每只大鼠右后足趾皮内注射0.lml弗氏完全佐剂,致炎后各组大鼠连续给药22天,采用酶联免疫吸附法测定大鼠血清IL-1β、IL-6、IL-10、TNF-α、NO、PGE2含量,免疫组化法检测滑膜组织中NF-κB的蛋白表达水平。结果:与模型组比较,刺三甲(7g/kg)可抑制AA大鼠的原发与继发性足肿胀,明显降低AA大鼠血清中IL-1β、IL-6、TNF-α、NO、PGE_2的含量和升高IL-10的含量,降低关节滑膜组织NF-κB的蛋白含量。结论:刺三甲可显著改善佐剂性关节炎大鼠的滑膜病变,通过下调滑膜组织NF-κB的蛋白表达。Objective： To observe effect of Acanthopanax trifo ethanol-extracts on the protein expression of NF-κB in ankle synovial tissue of AA rat. Methods： The 60 SD rats were randomly divided into six groups ： control group, model group, nimesulide group, Aeanthopanax 7.0g/ kg, 3.5g/kg and 1.75g/kg dose group of adjuvant arthritis rat, each rat posterior right toe of intradermal injection 0. lml Freundg complete adjuvant, after inflammation, the rats administered continuously for 22 days. The rat＇s model of adjuvant arthritis was established, the con- tents of IL-1β, IL-6, IL-10, TNF-ot, NO, PGE2 in rat serum were measured by ELLSA, detect the protein expression level of NF-κB in syn- ovial tissue through immunohistoehemical method. Results： Compared with model group, Acanthopanax trifo group（7g/kg） could obviously decrease the contents of IL-113, IL-6, TNF-α, NO, PGE2, increase the content of IL-IO, decrease the protein expression level of NF-KB in synovial tissue of ankle. Conclusion ： Aeanthopanax trifo has a obvious anti-inflammatory action to AA rat, its mechanmism of action may be related to down-regulating the protein expression of NF-κB in synovial tissue.

关 键 词：刺三甲 核转录因子-ΚB 白细胞介素 肿瘤坏死因子-α 一氧化氮 前列腺素E2 

分 类 号：R285.5[医药卫生—中药学]