浮游植物叶绿素a的微波法研究及其与反复冻融法的比较  

Microwave-assisted method for extracting chlorophyll-a in phytoplankton and its comparison with freezing-thawing extraction method

作　　者：林罗敏 唐鹊辉 彭亮[1] 韦桂峰[1] 林叔忠[2] 李湘姣[2] 杨浩文[2] 

机构地区：[1]暨南大学生态学系,热带亚热带水生态工程教育部工程研究中心,广州510632 [2]广东省水文局,广州510150

出　　处：《湖泊科学》2016年第5期1148-1152,共5页Journal of Lake Sciences

基　　金：广东省水利科技创新项目“广东省河流水生态健康评价指标体系及评价方法研究”(2014-01)资助

摘　　要：为缩短样品处理时间和提高测定准确度,本文设计和优化了微波处理辅助提取浮游植物叶绿素a的方法,并比较了反复冻融法和微波法对浮游植物叶绿素a的提取效率.结果表明：（1）微波法提取叶绿素a的最优处理条件为：高火（额定输出功率800 W）处理60 s左右.过滤水量为：贫营养型水体为1000 ml以上,中、富营养水体为100~500 ml.（2）反复冻融法在测定贫营养型水体时更稳定,而微波法对中、富营养水样提取率显著高于冻融法,可提高7%~12%,对具胶被及硅质外壳的藻类提取效率极显著高于冻融法,测定结果的相对偏差更小,且提取时间较冻融法缩短一半以上,适用于富营养化水体的应急监测.In order to save time of sample processing and to improve the determining accuracy, a microwave-assisted extraction method was designed and optimized to extract chlorophyll-a in phytoplankton, and was compared to the freezing-thawing method for extraction efficiency of chlorophyll-a. The results showed that microwave-assisted extraction method was optimal in 60 s treatment with output power of 800 W, and the appropriate water volume for filtration was about 100-500 ml for samples collected in mesoeutrophic waterbodies and 1000 ml for those from oligotrophic waterbodies. The chlorophyll extraction rate of microwave-assisted extraction method was significantly higher than that of freezing-thawing method in meso-eutrophic waterbodies, which can increased by 7%- 12%, while freezing-thawing method was more stable in measuring chlorophyll-a of oligotrophic waterbodies. For those algae with a glue shell or siliceous shell, microwave-assisted extraction method had a higher efficiency for chlorophyll-a extraction and a lower relative standard deviation than the freeze-thaw method. The microwave-assisted extraction method needs only one half operation time of the freezing-thawing extraction method, so that it is more suitable for emergency monitoring of eutrophic waterbodies, while the freezing-thawing extraction method is more suitable for monitoring oligotrophic waterbodies.

关 键 词：微波法 反复冻融法 提取 浮游植物 叶绿素A 

分 类 号：X832[环境科学与工程—环境工程]