中国北京男男同性恋艾滋病感染者HLA分型及与疾病进展分析  

Frequency of HLA gene in MSM cohort and correlation with AIDS disease progression

作　　者：孙坚萍[1] 孙焕芹[1] 刘宁[1] 刘桂海[1] 丛佳[2] 张永宏[1] 

机构地区：[1]首都医科大学附属北京佑安医院,北京100069 [2]首都医科大学附属北京同仁医院血液科,北京100730

出　　处：《中国免疫学杂志》2016年第8期1183-1186,1191,共5页Chinese Journal of Immunology

基　　金：国家十二五传染病重大专项(2012ZX10001006-001-008;2013ZX10001004-001-002);北京市科委(D131100005313004;D131100005313005;D141100000314005和D141100000314002)

摘　　要：目的:分析中国北京男男同性恋艾滋病感染者HLA-A、HLA-B、HLA-C基因频率以及HLA基因分型与疾病进展关系。方法:序列特异性引物的PCR扩增(PCR-sequence specific primer typing,PCR-SSP)对310名随机中国北京男男同性恋艾滋病感染者HLA-A、HLA-B、HLA-C基因进行分型,采用HIV Molecular Immunology Database中HLA Analysis Tools方法计算等位基因频率。结果:在北京男男同性恋艾滋病感染者队列中,HLA-A*1101(34.52%)基因频率最高,其次为HLA-A*0201(31.94%)、HLA-C*0102(27.10%)以及HLA-A*2402(26.45%)。根据艾滋病感染者1年内CD4细胞计数变化来判断感染者是否为快速进展者,结果发现快速进展者134例,非快速进展者176例。快速进展者中存在低水平的HLA-B*4403(快速进展者1.12%,非快速进展者为4.27%,P=0.0276),HLA-B*1511(非快速进展者5.98%,快速进展者2.25%,P=0.0282),HLA-B*5701(非快速进展者2.56%,快速进展者0.37%,P=0.0491)表达,而HLA-C*0304则在快速进展者中高表达(非快速进展者4.56%,快速进展者8.96%,P=0.0319)。结论:本研究获得了中国北京男男同性恋艾滋病感染者HLA-A、HLA-B、HLA-C基因的等位基因分布频率数据,并发现HLA-B*4403、HLA-B*1511、HLA-B*5701与延缓艾滋病疾病进展有关,而HLA-C*0304则与加速艾滋病疾病进展相关。Objective： HIV-1 infection was associated with a variety of host genetic factors,and HLA was one of the factors that contribute to the progression of disease. We analyze the frequency of HLA-A,HLA-B,HLA-C in MSM cohort,and the relationship between HLA gene with disease progression. Methods： PCR-sequence specific primer typing HLA typing was used to detect the allele frequency of HLA gene in 310 patients. HIV Molecular Immunology Database（ HLA Analysis Tools） to analysis the frequency of HLA. Results： In MSM cohort,HLA-A＊ 1101（ 34. 52%） gene was the highest,followed by HLA-A＊ 0201（ 31. 94%）,HLA-C＊ 0102（ 27. 10%） and HLA-A＊ 2402（ 26. 45%）. According to the CD4 cell count of HIV infected patients,the results showed that there were low levels of HLA-B＊ 4403（ 1. 12%,P = 0. 0276）,HLA-B＊ 1511（ 2. 25%,P = 0. 0282） and HLA-B＊ 5701（ 0. 37%,P = 0. 0491） in rapid progressors,while the HLA-C＊ 0304（ 8. 96%,P = 0. 0319） was higher than that of nonprogressors（ 4. 56%）. Conclusion： We obtained the distribution frequency data of HLA-A,HLA-B and HLA-C in MSM cohort. Our data presented here may offer potential correlation between dominant effect of HLA alleles and HIV-1 disease progression. And found that the HLA-B ＊ 4403,HLA-B ＊ 1511,HLA-B＊ 5701 related to slow HIV disease progression and HLA-C＊ 0304 related to accelerate HIV disease progression.

关 键 词：HIV 人类白细胞抗原 男男同性恋 

分 类 号：R512.91[医药卫生—内科学]