肺炎支原体肺炎患儿外周血CXCL8及其mRNA表达  

Expression of CXCL8 and its mRNA in peripheral blood of children with Mycoplasmal pneumonia

作　　者：卜小芳 王健[2] 倪宁[1] 田恒忠 祁庆松 孔占一 

机构地区：[1]安徽省淮南市妇幼保健院儿科,淮南232007 [2]安徽理工大学医学院病原学与免疫学教研室,淮南232001

出　　处：《中国免疫学杂志》2016年第8期1195-1199,共5页Chinese Journal of Immunology

基　　金：安徽省自然科学基金(No.1308085MH148);安徽省教育厅自然科学基金重点项目(No.KJ2010A086);安徽省教育厅重大自然科学研究项目(No.KJ2016SD20);淮南市2015年科技计划项目(No.2015A2406)

摘　　要：目的：探讨支原体肺炎患儿外周血CXCL8及其mRNA表达的临床意义。方法：收集2013年10月～2015年3月淮南市妇幼保健院收治的支原体肺炎患儿48例,其中重症12例,轻症36例,以ELISA法检测患儿血清CXCL8含量,PCR法检测患儿外周血单个核细胞内CXCL8 mRNA水平。以GAPDH为参照,以lgc DNA/lg GAPDH比值代表其最终mRNA水平。结果：支原体肺炎患儿外周血血清CXCL8含量及外周血单个核细胞内CXCL8 mRNA水平分别为（298.917±51.860）pg/ml、（1.848±0.525）lgc DNA/lg GAPDH,与正常对照相比差异均有显著统计学意义（P〈0.05）。进一步观察发现,重症患儿外周血CXCL8及其mRNA进一步升高,与轻症组相比,血清CXCL8含量差异无显著统计学意义统计学意义（P〉0.05）,而CXCL8mRNA水平差异有显著统计学意义（P〈0.05）。急性期以红霉素静脉注射7～10 d,使患儿病情得以明显控制,咳嗽症状减轻,肺部炎症逐渐改善,病情得到有效控制,再以阿奇霉素序贯治疗2～3周,患儿病情逐步由急性期转为恢复期,此时患儿外周血CXCL8及其mRNA水平明显降低,与急性期相比,差异有显著统计学意义（P〈0.05）。结论：支原体肺炎患儿外周血CXCL8及其mRNA表达水平增高,并与病情的严重程度相关。CXCL8参与支原体肺炎的发病过程,并对病情的轻重程度和转归有一定的提示作用。阿奇霉素可通过抑制肺炎支原体增殖途径降低患儿血清中CXCL8含量、下调CXCL8 mRNA的表达,逐渐抑制由肺炎支原体介导的免疫损伤。Objective： To study the expression of CXCL8 in the serum and CXCL8 mRNA in the peripheral blood mononuclear cells（ PBMCs） of the children with Mycoplasma pneumoniae pneumonia（ MPP） and its clinical significance. Methods： Forty-eight children（ severe cases 12,light cases 36） with MPP were recruited from October 2013 to March 2015 in the Maternal and Child HealthCare Hospital of Huainan. The concentration of the CXCL8 in serum and the level of CXCL8 mRNA in the PBMCs were measured by enzyme linked immunosorbent assay（ ELISA） and polymerase chain reaction（ PCR）. Taking GAPDH as the internal reference,the ratio of lgc DNA / lg GAPDH was regarded as the extreme level of CXCL8 mRNA. Results： The serum level of CXCL8 and expression of CXCL8 mRNA in PBMCs in the children with MPP were（ 298. 917 ± 51. 860） pg / ml and（ 1. 848 ± 0. 525） lgc DNA / lg GAPDH. Compared with the normal control,there were significant differences between the two groups（ P〈0. 05）. Further observation showed that the levels of CXCL8 in serum were no significant difference between in light cases and severe illness（ P〉0. 05）. However,the expression of CXCL8 mRNA in peripheral blood of the children with severe illness was significantly higher than those in light cases（ P〈0. 05）. Intravenous infusion of Erythromycin was provided in the acute phase for seven to ten days,so that the children＇ s condition could be significantly controlled,and the symptoms of pulmonary inflammation were also relieved. Followed by the use of sequential therapy of Azithromycin for about two to three weeks,the children＇s condition were gradually from acute stage to recovery stage. At this time,the CXCL8 and its mRNA levels in peripheral blood of the sick children were all significantly decreased comparing with those in the acute stage（ P〈0. 05）. Conclusion： The expression of CXCL8 and its mRNA were increased in the peripheral blood of the sick children with Mycoplasma pneumonia,and also correlated with the seve

关 键 词：肺炎支原体 支原体肺炎 外周血单个核细胞 CXCL8 mRNA 

分 类 号：R375.2[医药卫生—病原生物学] R392.11[医药卫生—基础医学]