美沙拉秦、酪酸梭菌、蒙脱石散对UC大鼠血中PG-E2、LT-B4、PAF、VEGF的影响  

Impacts of mesalazine,clostridium and montmorillonite powder on plasma PG-E2,LT-B4,PAF and VEGF in rats with ulcerative colitis

作　　者：何山[1] 刘凯[1] 黄永坤[2] 

机构地区：[1]云南省第一人民医院儿科,昆明650000 [2]昆明医科大学附属第一医院儿科,昆明650000

出　　处：《中国免疫学杂志》2016年第8期1212-1218,共7页Chinese Journal of Immunology

摘　　要：目的:探讨模型UC大鼠血中前列腺素-E2(PG-E2)、白三烯B4(LT-B4)、血小板活化因子(PAF)及血管内皮生长因子(VEGF)的水平,及经过美沙拉秦、酪酸梭菌、蒙脱石散治疗干预后上述四个因子的变化情况,以了解这四个炎性因子在模型UC大鼠血中的意义及上述三种药物对这四个炎性因子的干扰效果。方法:将100只大鼠随机分成正常组、模型组、美沙拉秦组、酪酸梭菌组、蒙脱石散组,用酶联免疫吸附试验分别检测各组小鼠血中第12天血中的PG-E2、LT-B4、PAF、VEGF的含量,并进行统计学分析。结果:1模型组、美沙拉秦组、酪酸梭菌组、蒙脱石散组较正常组的DAI及血清PG-E2、LT-B4、PAF、VEGF含量均升高,差异有统计学意义(P<0.05)。2与模型组比较,美沙拉秦组、酪酸梭菌组、蒙脱石散组的DAI及血清PG-E2、LT-B4、PAF、VEGF含量均降低,差异有统计学意义(P<0.05)。3美沙拉秦、酪酸梭菌、蒙脱石散的治疗效果比较,美沙拉秦及蒙脱石散疗效较好,差异有统计学意义(P<0.05),美沙拉秦与蒙脱石散疗效无差异。结论:1在TNBS/乙醇法诱导的UC大鼠血清中PG-E2、LT-B4、PAF、VEGF表达增高,与炎症呈正相关。2美沙拉秦、酪酸梭菌、蒙脱石散可下调模型大鼠促炎因子PG-E2、LT-B4、PAF、VEGF的表达,这可能与UC大鼠肠道炎症的缓解,促进大鼠UC的恢复有关。Objective： To investigate the Prostaglandin E2（ PG-E2）,Leukotrienes B4（ LT-B4） 、Platelet activating factor（ PAF）and vascular endothelial growth factor（ VEGF） levels in the model of UC rats serum,and the changes after the Mesalazine,clostridium,and montmorillonite powder treatment intervention,in order to understand the significance of the four inflammatory factors in UC rats and the interference effect of the above three drugs on the four inflammatory cytokines. Methods： 100 rats were randomly divided into normal group（ A）,model group（ B）,Mesalazine group（ C）,clostridium group（ D） and montmorillonite group（ E）,The levels of the plasma PG-E2,LT-B4,PAF and VEGF were measured by ELISA. The different changes of the four cytokines were compared in the different groups. Results： 1The scores of DAI and the levels of the plasma PGE2,LTB4,PAF and VEGF in the B,C,D and E group were higher than ones in A group（ P〈0. 05）. 2Compared B group,the scores of DAI and the levels of plasma PGE2,LT-B4,PAF and VEGF in B,C,D and E group were lower（ P〈0. 05）. 3 Compared the efficacy among mesalazine,clostridium and montmorillonite powdergot treating UC,mesalazine and montmorillonite powder got the best results（ P〈0. 05）. The efficacy between mesalazine and montmorillonite was similar. Conclusion： 1The PGE2,LT-B4,PAF and VEGF are very active higher in the blood of rats with UC and have a positive correlation with inflammation. 2Mesalazine,clostridium and montmorillonite powder may renovate the damage of inflammatory tissues of rats with UC,and relieve the symptoms of inflammation,and also shorten the duration of inflammation by reducing exudation of proinflammatory cytokines PGE2,LTB4,PAF and VEGF.

关 键 词：美沙拉秦 酪酸梭菌 蒙脱石散 溃疡性结肠炎 大鼠 细胞因子 

分 类 号：R547[医药卫生—心血管疾病]