环磺酮原药的反相高效液相色谱分析  

Determination of Active Ingredients in Tembotrione TC by RP-HPLC

作　　者：黄成田[1,2] 丁琦[1] 纪明山[2] 李莹[1] 贾福艳[1] 周银平[1] 龚党生[1] 胥维昌[1] 

机构地区：[1]沈阳化工研究院有限公司,沈阳110021 [2]沈阳农业大学植物保护学院,沈阳110866

出　　处：《农药》2016年第9期670-671,共2页Agrochemicals

摘　　要：【目的】建立一种反相高效液相色谱测定环磺酮原药的定量分析方法。【方法】采用高效液相色谱法，使用AgilentExtend—C18色谱柱，以乙腈-0．1％磷酸溶液（体积比为50：50）为流动相，流速为0．8mL／min，紫外检测器检测波长为285nm，对环磺酮原药进行分离和测定。【结果】当环磺酮质量浓度为40．0～1000mg／L时，环磺酮的线性相关系数为0．99983，标准偏差为0．21，变异系数为0．22％，平均回收率为100．05％。【结论】该方法准确，快速，分离效果好，适合于环磺酮原药的定量分析。[Aims] A RP-HPLC method for the quantitative determination of tembotrione TC was established. [Methods] The quantitative analysis of tembotrione TC was carried out by using Agilent Extend-C18 column with the mixture solution ofacetonitrile and 0.1% phosphoric acid （50：50, by vol） as mobile phase at the flow rate of 0.8 mL/min. The wavelength of UV detector was 285 ran. [Results] When the concentration oftembotrione was ranged from 40.0 to 1000 mg/L, the linear correlation coefficient was 0.999 83, the standard deviation was 0.21, the variation coefficient was 0.22%, and the mean recovery was 100.05%. [Conclusions] The method is simple, accurate and fast, which is suitable for quantitative analysis oftembotrione TC.

关 键 词：环磺酮 高效液相色谱 定量分析 

分 类 号：O657.72[理学—分析化学] TQ457[理学—化学]