磷脂酰胆碱组分的二维液相色谱分离  

Analysis of the Phosphatidyl Cholines from Halichondria Rugosa Cell Membrane by Off-line Two-dimensional Liquid Chromatography

作　　者：郝丽[1] 朱苑露 代雅楠 段蓿 韩欣欣[1] 谭成玉[1] 孔亮[1] 李伟[1] 

机构地区：[1]大连海洋大学,辽宁大连116023

出　　处：《农产品加工》2016年第8期56-58,共3页Farm Products Processing

基　　金：海洋局公益性行业科研专项(201005024-3;201205022-7)

摘　　要：建立了以脂质体生物色谱-反相高效液相色谱的离线二维色谱分析方法模型,以磷脂酰胆碱(Phosphatidyl choline,PC)为样品进行色谱分析。首先采用PBS(磷酸盐缓冲溶液)为流动相,流速为0.4 m L/min,以固定相脂质体液相色谱柱(Immobilized lliposomes chromatography,ILC)的一维色谱分离。二维色谱分离采用反相C_(18)色谱柱进行分离,流速为1 m L/min。磷脂酰胆碱在固定相脂质体色谱上仅有1个保留峰,但在反相C_(18)色谱柱上却存在着多个保留峰。因此,由脂质体生物色谱-反相高效液相色谱组成的二维分离色谱在阐释了生物色谱意义的同时也充分体现了二维色谱的分离优越性。In this paper, phosphatidyl cholines （PCs） in Halichondria Rugosa Cell Membrane are analyzed by the model of immobilized liposomes chromatography （ILC） -RP-HPLC （Off-line two-dimensional liquid chromatography） . Research has revealed that PC retained peaks on liposome chromatography stationary phases and separate well on the RP-HPLC C18- Using established PC model the phospholipid compounds of the marine sponge cell membrane is validation. The results show that he main components of cell membrane are carbohydrates, DNA and proteins have been removed by the first dimensional chromatography （ILC）, and the PCs can be separated by the second dimensional chromatography （RP-HPLC） quickly.

关 键 词：固定化脂质体色谱 二维色谱 磷脂酰胆碱 

分 类 号：Q545.1[生物学—生物化学]