沙利度胺联合达那唑对子宫内膜异位症裸鼠异位组织血管生成及血管内皮生长因子的影响  

Effect of thalidomide combined with danazol on angiogenesis and vascular endothelial growth factor of ectopic tissues in nude mice with endometriosis

作　　者：李宁[1] 苏芳 宁海丹 彭兴[4] 尧良清[5] 黄玉葵[1] 范余娟[6] 

机构地区：[1]广西南宁市妇幼保健院妇科,南宁市530001 [2]广西南宁市第三人民医院妇产科,南宁市530003 [3]广西壮族自治区民族医院病理科,南宁市530001 [4]广西医科大学药学院,南宁市530021 [5]复旦大学附属妇产科医院妇科,上海市200011 [6]广西医科大学第一附属医院妇科,南宁市530021

出　　处：《广西医学》2016年第9期1207-1210,共4页Guangxi Medical Journal

基　　金：广西科学研究与技术开发计划(桂科攻12300016);广西南宁市科学研究与技术开发计划(20133174)

摘　　要：目的探讨沙利度胺联合达那唑对子宫内膜异位症裸鼠异位组织血管生成及血管内皮生长因子(VEGF)的影响。方法选择40只成功建立子宫内膜异位症模型的裸鼠,随机分为模型对照、沙利度胺组、达那唑组及联合用药组,每组10只,分别给予生理盐水、沙利度胺30 mg/(kg·d)、达那唑1.0 mg/(kg·d)、沙利度胺30 mg/(kg·d)联合达那唑1.0 mg/(kg·d)腹腔注射,给药4周后处死,通过Ⅷ因子标记异位子宫内膜血管,检测异位内膜组织中微血管密度(MVD),链霉菌抗生物素蛋白-过氧化物酶法测定异位内膜VEGF蛋白的表达情况。结果对照组、达那唑组、沙利度胺组和联合用药组的MVD依次降低(P<0.05),分别为(21.3±1.5)个、(16±3.4)个、(12.2±1.1)个和(6.8±1.1)个。对照组、达那唑组、沙利度胺组和联合用药组VEGF蛋白的表达水平也依次降低(P<0.05)。结论沙利度胺和达那唑可抑制裸鼠异位内膜的MVD以及VEGF的表达,从而抑制异位内膜组织的血管生成,两者合用时作用更明显。Objective To investigate the effect of thalidomide combined with danazol on angiogenesis and vascular endothelial growth factor（VEGF） of ectopic tissues in nude mice with endometriosis. Methods Forty nude rats with successful modeling of endometriosis were randomly divided into control group, thalidomide group, danazol group and combined administration group, with 10 mice in each group. The control group,thalidomide group,danazol group and combined administration group were administered normal saline,halidomide [ 30 rag/（kg · d） ], danazol[ 1.0 mg/（kg· d） ] and halidomide[ 30 mg/（kg· d） ] combined with danazol[ 1.0 mg/（kg·d） ]by intraperitoneal injection respectively. All rats were killed after four weeks of administration. The ectopic endometrial vessels were marked by Ⅷ factor,and then microvessel density（MVD） of ectopic endometrium tissues was detected. The expression of ectopic endometrial VEGF was determined by streptavidin-peroxidase assay. Results MVDs decreased in the order of control group （21.3 ±1.5 ）, danazol group （ 16 ± 3.4）, thalidomide group （ 12.2 ± 1.1 ） and combined administration group（6.8 ± 1.1 ） （ P 〈 0.05 ）. The expression levels of VEGF protein decreased in the order of control group, danazol group, thalidomide group and combined administration group （ P 〈 0.05 ）. Conclusion Thalidomide and danazol can inhibit the MVD and the VEGF expression of ectopic endometrium in the nude mice,thus can inhibit the angiogenesis in ectopic endometrium. Thalidomide combined with danazol can obtain more obvious inhibitory effect.

关 键 词：子宫内膜异位症 沙利度胺 达那唑 血管内皮生长因子 微血管密度 裸鼠 

分 类 号：R711.71[医药卫生—妇产科学]