雷公藤红素对IgA肾病大鼠肾脏组织中Notch信号通路表达的影响研究  

Effect of celastrol on the expression of Notch signaling pathway in rats with IgA nephropathy

作　　者：刘丹[1] 王彩丽[1] 

机构地区：[1]包头医学院第一附属医院肾内科,内蒙古包头014010

出　　处：《重庆医学》2016年第25期3457-3461,共5页Chongqing medicine

摘　　要：目的探讨雷公藤红素对IgA肾病大鼠肾脏组织中Notch信号通路表达的影响。方法建立IgA肾病大鼠模型,观察雷公藤红素治疗后大鼠血尿、蛋白尿情况,采用免疫组织化学和实时荧光定量PCR检测Notch1、Jagged1及其下游靶基因Hes1、Hey1的表达。结果与对照组比较,IgA肾病模型组血尿及24h尿蛋白定量明显增高(P<0.05);第16、20周末与模型组比较,贝那普利组及雷公藤红素干预组血尿、24h尿蛋白定量明显下降(P<0.05),且贝那普利组及高剂量组较低剂量组下降明显(P<0.05)。各组大鼠尿素氮(BUN)、血肌酐(Scr)、谷丙氨基酸转移酶(ALT)、谷草氨基酸转移酶(AST)比较,差异无统计学意义(P>0.05)。对照组大鼠肾小球、肾小管细胞质中Notch1、Jagged1仅有少量表达,在模型组大鼠肾脏组织的肾小球及肾小管细胞质中表达明显增加,贝那普利组、低剂量组、高剂量组Notch1、Jagged1的表达较模型组减弱,但仍高于对照组。而Hes1、Hey1在对照组大鼠肾小球、肾小管细胞质中基本无表达,在模型组大鼠肾脏组织的肾小球细胞核表达增加,贝那普利组、低剂量组、高剂量组Hes1、Hey1的表达较模型组减弱,但仍高于对照组。结论雷公藤红素可以通过抑制IgA肾病大鼠肾组织中Notch信号通路的表达,减少血尿、蛋白尿的生成,从而对大鼠IgA肾病起到治疗作用。Objective To investigate the effect of celastrol on expression of Notch signaling pathway in renal tissues of IgA nephropathic rats. Methods Rat model of IgA nephropathy was established to Observe the hematuria and proteinuria statuses in rats after celastrol treatment. Expression levels of Notch1 ,Jaggedl and downstream target genes Hesl, Heyl were detected by im- munohistochemistry and real-time fluorescence quantitative PCR. Results Compared with control gr0up, hematuria and 24 hour u- rinary protein quantitative in IgA nephropathy model group was significantly increased （P^0.05） ;At the end of sixteenth, the 20 week, compared with the model group, hematuria, 24 hour urinary protein quantitative in benazepril group and tripterine interven- tion group were decreased significantly（P%0.05） ,compared with low dose group ,benazepril group and high dose group were de- creased significantly（P%0.05）. The difference of urea nitrogen （BUN）, serum creatinine （Scr）, alanine aminotransferase （ALT） and aspartate aminotransferase （AST） among groups had no statistically significant（P^0.05）. The Notch1 Jagged1 in glomerular and renal tubular cells in cytoplasm of control group had only a small amount of expression, the expression of Notchl and Jagged1 in glomerular and tubular cells in the renal tissue of the rats in the model group were significantly increased; compared with the model group,the expression of Notchl, Jagged1 in benazepril group, low dose group, high dose group were decreased, but still higher than the control group. While Hesl and Heyl in glomerular and renal tubular cell nucleus in cytoplasm of model group had no expression , the expression increased in glomerular cell nucleus of the model group, the expression of Notch1, Jagged1 in benaze- pril group, low dose group, high dose group were decreased, but still higher than the control group. Conclusion Celastrol can re- duce the production of hematuria and proteinuria by inhibiting the expression of Notch signaling pathway in ren

关 键 词：肾小球肾炎 IGA 大鼠 NOTCH信号通路 雷公藤红素 

分 类 号：R285.5[医药卫生—中药学]