PCR检测贵州遵义地区蝙蝠BDV-P40基因片段及同源性分析  

PCR detection on the P40 gene fragment of borna disease virus in bats and its homology analysis in Zunyi area of Guizhou province

作　　者：雷以会 徐平[1] 杨利玲[1] 

机构地区：[1]遵义医学院附属医院神经内科,贵州遵义563000

出　　处：《重庆医学》2016年第25期3475-3477,共3页Chongqing medicine

摘　　要：目的探讨贵州遵义地区蝙蝠是否存在博尔纳病毒(BDV)自然感染,分析比较其与国外标准株的同源性。方法采用实时定量逆转录-聚合酶链反应(RT-PCR)检测82只蝙蝠额颞叶脑组织中BDV-P40基因片段,将阳性扩增产物测序,与国外标准株比较同源性。结果蝙蝠额颞叶脑组织中BDV-P40基因片段阳性率为12.2%(10/82)。贵州遵义地区蝙蝠自然感染的BDV-P40核苷酸序列与标准株Strain V和H1766同源性均为96.2%,与He/80比对同源性为94.9%,所编码的氨基酸无改变。结论贵州遵义地区蝙蝠存在BDV自然感染,该地区BDV-P40核苷酸序列与Strain V和H1766存在高度同源性,人感染BDV可能为动物源性。Objective To investigate whether the bat in Zunyi region of Guizhou province exist Borna disease virus（BDV） natural infection,and to explore the homology with the foreign standard virus strains. Methods BDV-P40 in frontal and temporal lobe brain tissue of 82 bats were detected with real-time reverse transcription polymerase chain reaction （RT-PCR）, the BDV-P40 positive samples were sequenced and compared with the foreign standard virus strains. Results The BDV-P40 positive rate was 12.2% （10/82）in frontal and temporal lobe brain tissue of bats. After sequence comparison,BDV-p40 fragment of the nucleotide se- quence in Strain V, H1766 showed 96.2 % homology, He/80 exhibited 94.9% homology, but there were no change in the encoding amino acid residues. Conclusion The bats in Zunyi region of Guizhou province exist latent infection of BDV,and the positive BDV- P40 nucleotide sequence is highly homologous with strain V and H1766,human infection with BDV may be a source of animals.

关 键 词：博尔纳病毒 实时定量逆转录-聚合酶链式反应 蝙蝠 BDV-P40基因片段 

分 类 号：Q78[生物学—分子生物学] R440[医药卫生—诊断学]