肿瘤微环境中TGFβ-1对子宫内膜间质细胞活化的影响  

Effects of TGF β-1 on the activation of endometrial stromal cells in tumor microenvironment

作　　者：路真真 颜磊[1] 张辉[1] 张晓晖[1] 赵兴波[1] 

机构地区：[1]山东大学附属省立医院妇产科,山东济南250021

出　　处：《山东大学学报（医学版）》2016年第9期37-40,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81272858)

摘　　要：目的探讨子宫内膜癌肿瘤微环境及转化生长因子β-1(TGFβ-1)对正常间质细胞(NF)活化为肿瘤相关成纤维细胞(CAF)的影响。方法 1体外培养人子宫内膜癌细胞系Ishikawa细胞,制备条件培养基(CM),原代培养NF,形成共培养体系,Western blotting与QT-PCR方法检测其α-平滑肌肌动蛋白(α-SMA)的表达。2分组为:A:NF,B:NF+TGFβ-1,C:NF+CM,D:NF+CM+TGFβ-RⅠ/Ⅱ激酶抑制剂。结果 B和C组α-SM A蛋白表达较A组高(P=0.01、P<0.001),B和C组α-SMA的mRNA表达较A组高,两组比较差异有统计学意义(P<0.001、P=0.01);B和C组α-SM A蛋白表达较D组高(P<0.001、P=0.01),B和C组α-SM A的mRNA表达较D组高,两组比较差异有统计学意义(P<0.001、P=0.01)。与C组相比,B组α-SM A蛋白表达较C组高(P<0.001)。B组与C组比较α-SM A mRNA表达差异无统计学意义(P=0.40)。结论子宫内膜癌肿瘤微环境可促进NF-CAF的转化,TGFβ-1在该过程中发挥重要作用。Objective To investigate the effects of endometrial carcinoma microenvironment and transforming growth factor β-1 （TGF β-1 ） on the activation of normal endometrial fibroblasts （NF） to carcinoma-associated fibroblasts （CAF）. Methods Endometrial carcinoma cell line （Ishikawa cells ） was cultured in vitro. Conditioned medium （CM） was prepared. Primarily cultured NF was co-cultured. The cells were divided into four groups and treated accordingly： NF group （group A）, NF ＋TGF β1 （group B）, NF ＋CM （group C）, and NF ＋CM ＋TGF β-R I/II kinase inhibitors （group D）. The expression of a-smooth muscle actin （a-SMA） was detected with QT-PCR and Western blotting. Results The a-SMA protein and mRNA expressions in group B and C were higher than those in group A （P =0. 01, P 〈 0. 001; P 〈 0. 001, P = 0.01 ）. The a-SMA protein and mRNA expressions in group B and C were higher than those in group D （P 〈0. 001, P =0. 01 ; P 〈0. 001, P =0.01 ）. Compared with group C, group B had higher a-SMA protein expression （ P 〈 0.001 ）, but the a-SMA mRNA expression had no statistical difference （ P = 0.40 ）. Conclusion Endometrial carcinoma microenvironment can promote the transformation of NF-CAF, and TGFβ-1 plays an important role in the process.

关 键 词：肿瘤相关成纤维细胞 子宫内膜癌 肿瘤微环境 Α-平滑肌肌动蛋白 

分 类 号：R711.74[医药卫生—妇产科学]