纯镁超声微弧氧化-NaOH-硅烷-丝素复合处理膜层的细胞相容性  

Biocompatibility of Pure magnesium with synergy treatment of micro-arc oxide,NaOH alkali and silane-silk fibroin modification

作　　者：刘明达[1] 段峰[1] 李慕勤[1] 裴魏魏 陈洪伟[1] 王爽[1] 

机构地区：[1]佳木斯大学黑龙江省高校生物医学材料重点实验室,佳木斯154007

出　　处：《中国体视学与图像分析》2016年第2期206-212,共7页Chinese Journal of Stereology and Image Analysis

基　　金：佳木斯大学研究生科技创新项目(LM2015_058);国家自然科学基金(No.31370979);黑龙江省高校创新团队建设计划项目;生物医用材料及其表面改性(2012TD010)

摘　　要：目的针对纯镁微弧氧化膜层腐蚀和活性弱问题,本文对纯镁材料进行超声微弧氧化膜层结合Na OH-硅烷-丝素蛋白复合处理,探讨不同处理膜层对成骨细胞黏附、增殖的影响,为新型医用植入材料应用提供临床研究依据。方法超声微弧氧化处理(简称UMAO)作为对比组(A组),UMAO-Na OH-硅烷后,丝素浸渍处理时间分别30 min(B组)和90 min(C组)为实验组,上述材料进行成骨细胞的生长、黏附、增殖实验研究。利用扫描电镜和激光共聚焦显微镜表征膜层表面形貌和细胞早期形态。利用CCK-8和ALP分别检测成骨细胞增殖情况和活性。结果实验组表面成骨细胞的生长和黏附均优于A组,并且C组的结果优于B组。三组材料表面上细胞的增殖、活性为C组>B组>A组,三组比较差异性均具有统计学意义。结论纯镁材料经UMAO膜层结合Na OH-硅烷-丝素蛋白复合处理后,其生物相容性有所改善,而经复合处理结合丝素蛋白浸渍90 min后的纯镁膜层生物相容性最佳。Objective In view of the weak corrosion resistance and low activity of micro-arc oxide coatings for pure magnesium materials, this paper developed a new technology of micro-arc oxidation, NaOH alkali treatment and surface modification with silane-silk fbroin flms. The effect of different treatments on osteoblast adhesion and proliferation behaviors were investigated in detail. This new method will provide reference for new medical implant materials. Methods Three groups of materials were set up： contrast group（group A） was materials treated with ultrasonic micro-arc oxidation, experimental groups（Group B and C ） were materials treated with immersion in silk fibroin solution for 30 min and 90 min respectively after micro-arc oxidation, NaOH alkali treatment and surface modification with silanization. The growth, adhesion and proliferation of osteoblasts for these materials are studied in our experiments. The film surface morphology and cell form in early stage were characterized by means of scanning electron microscope and laser scanning confocal microscope. The osteoblast proliferation and activity condition were investigated by CCK 8 and ALP methods. Results the growth and adhesion condition of osteoblasts for the experimental groups was better than that of the contrast group. In the meantime, Group C is of better condition in the experimental groups. The differences were statistically significant. Conclusions The biocompati-bility is improved after surface modified treatment of MAO coating together with NaOH-silane-silk fibroin combined treatment. The bioco combined treatment under the s mpatibility of the pure magnesium material with NaOH-silane-silk fbroin ilk fibroin dipping for 90 min was the best.

关 键 词：纯镁 微弧氧化处理 硅烷化处理 丝素蛋白浸渍 细胞 

分 类 号：R318.08[医药卫生—生物医学工程]