广西地区HIV合并HCV感染人群HCV基因型分布特征  

HCV genotype distribution in HIV/HCV co-infected patients in Guangxi

作　　者：陈旭[1] 苏明华[1] 江建宁[1] 韦智 朱明月[3] 周奕[1] 伍菲菲[1] 滕春玲[1] 

机构地区：[1]广西医科大学第一附属医院感染性疾病科,南宁530021 [2]防城港市第一人民医院感染性疾病科,广西防城港538021 [3]北海市人民医院感染性疾病科,广西北海536000

出　　处：《中国艾滋病性病》2016年第8期593-596,共4页Chinese Journal of Aids & STD

基　　金：广西教育厅基金资助项目(YB2014064);广西卫生厅基金资助项目(Z2011346);广西南宁青秀区科技局基金资助项目(2013ZH02)~~

摘　　要：目的了解广西地区艾滋病病毒(HIV)和丙型肝炎病毒(HCV)感染人群HCV基因型的分布特征。方法收集广西地区51例HCV RNA阳性HIV感染者的血清,通过对反转录巢式聚合酶链反应(RT nested-PCR)扩增HCV NS5B区段,所得终产物直接测序进行HCV基因分型。结果在51例样本中,HCV基因型以3b和6a型为主,各占23.53%(12/51),其次为1a型,占19.61%(10/51);3a型、1b型、6d型分别占13.73%(7/51)、9.80%(5/51)、1.96%(1/51),2a型、6e型各占3.92%(2/51)。分析表明,HIV合并HCV感染者的HCV基因在性别、年龄、感染途径分布差异没有统计学意义(P>0.05)。结论广西地区HIV合并HCV感染者流行的HCV基因亚型至少有1a、1b、2a、3a、3b、6a、6d、6e共8种,3b和6a可能为流行的主要基因亚型,1a型次之。初步说明广西地区HIV合并HCV感染人群流行的HCV基因亚型呈多样性,同时它们与性别、年龄及感染途径无明显相关性。Objective To investigate the genotype distribution of hepatitis C virus （HCV）among the patients in- fected with both human immunodeficiency virus （HIV） and HCV in Guangxi. Methods We collected a total of 51 confirmed both HCV RNA and HIV-positive samples. HCV genotypes and sub-genotypes were determined by direct sequencing of reverse transcription-polymerase chain reaction （RT-PCR） amplifiedfrom HCV NS5B region. Results HCV sub-genotype 3b and 6a were predominant, presenting 23.53% each, and followed by la accounting for 19.61%. 3a,lb and 6d were observed as 13.73% ,9. 80% and 1.96%, respectively. 2a and 6a each contributed to 3.92% among the total of the 51. The HCV genotype distributions according to the gender,age and transmission routs showed no significant difference in HIV/HCV co-infected individuals. Conclusion There are at least eight HCV sub-genotypes among the HIV/HCV co-infected individuals in Guangxi,including la, lb,2a,3a,3b,6a,6d and 6e. The main sub-genotypes are both 3b and 6a,followed by la. It indicates the diversity of the HCV genotypes, and the genotype distributions are not associated with gender,age or transmission routes among the HIV/HCV co-infec- ted patients in Guangxi.

关 键 词：广西地区 艾滋病病毒合并丙型肝炎病毒感染 基因型 

分 类 号：R512.91[医药卫生—内科学]