黄杆菌裂解产物对巨噬细胞源性泡沫细胞形成的作用及机制研究  

The effect of Flavobacterium lysates on the formation of macrophage-derived foam cell and the invovled mechanism

作　　者：李坑[1] 黄艳[2] 夏静鸿[1] 张雪冬[1] 靳丽妍[1] 朱光发[1] 

机构地区：[1]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所感染科,100029 [2]首都医科大学附属北京安贞医院-北京市心肺血管疾病研究所呼吸与危重症医学科,100029

出　　处：《心肺血管病杂志》2016年第8期659-663,共5页Journal of Cardiovascular and Pulmonary Diseases

基　　金：首都医科大学基础临床科研合作项目(15JL60)

摘　　要：目的:探讨黄杆菌裂解产物对巨噬细胞源性泡沫细胞形成的作用及可能的机制。方法:将黄杆菌裂解产物与THP-1细胞进行共培养,观察其对THP-1巨噬细胞及泡沫细胞形成的影响,并检测CD14、TLR4、LDLr三个受体mRNA的表达水平。结果:黄杆菌裂解产物促进THP-1单核细胞向巨噬细胞分化,并促进巨噬细胞源性泡沫细胞的形成,上调CD14、TLR4、LDLr三个受体mRNA的表达水平。结论:黄杆菌裂解产物促进单核巨噬细胞源性泡沫细胞的形成,这可能与其上调LDLr表达有关。Objective： To investigate the effect of Flavobacterium lysate on macrophage-derived foam cell formation and the possible mechanisms. Methods： Flavobacterium lysate and THP-1 cells were co-cultured,observe its effect on THP-1 macrophages and macrophage-derived foam cell formation,also detect the mRNA expression levels of CD14,TLR4,LDLr. Results： Flavobacterium lysates promote THP-1 monocyte differentiated into macrophage, and promote the formation of macrophage-derived foam cells, increased CD14,TLR4,LDLr mRNA expression. Conclusion： Flavobacterium lysate promotes the formation of macrophage-derived foam cells,which may increase its expression LDLr. Flavobacterium lysate may also up-regulate the expression of CD14 and TLR4 of the monocyte-macrophage cells involved in inflammation of atherosclerosis.

关 键 词：黄杆菌裂解产物 单核细胞 巨噬细胞 泡沫细胞 

分 类 号：R56[医药卫生—呼吸系统]