SrHA/PMMA复合骨水泥的制备、理化性能及细胞相容性研究  

Preparation,handling properties and cytocompatibility of SrHA/PMMA composite bone cement

作　　者：邓育红 崔旭[2] 彭松林 

机构地区：[1]深圳市人民医院脊柱外科,广东深圳518020 [2]中国科学院深圳先进技术研究院,广东深圳518055

出　　处：《生物骨科材料与临床研究》2016年第4期1-7,共7页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：深圳市科技创新委员会项目(JSGG20150331154931068;CXZZ20150401152251209)

摘　　要：目的结合PMMA和锶羟基磷灰石（Sr HA）各自的优势,制备出兼具高的力学强度、合适的固化时间、较低的热释放、生物活性和骨整合性能的Sr HA/PMMA复合骨水泥,并系统性地研究Sr HA的引入对复合骨水泥的体外固化性能、力学强度和生物学性能的影响。方法将水热合成法制备的锶羟基磷灰石引入PMMA基体,制备Sr HA/PMMA复合骨水泥。系统性地对Sr HA/PMMA复合骨水泥的力学强度、固化时间、热释放、生物活性进行研究。将复合骨水泥和细胞共培养,利用MTT法、扫描电镜等研究Sr HA/PMMA复合骨水泥的细胞毒性,粘附和增殖。结果结果表明,与纯的PMMA骨水泥（对照组）相比,Sr HA/PMMA复合骨水泥的固化热释放明显降低（约80-84℃）,同时又维持了合适的固化时间（8-11分钟）和较好的力学性能（抗压强度为90MPa左右）。Sr HA的引入,不仅赋予了复合骨水泥生物活性,也显著地改善了其细胞/材料的相互作用。浸泡在SBF后,Sr HA/PMMA复合骨水泥显示出更好的体外矿化性能。与成骨细胞MC3T3-E1共培养后,表面沉积的羟基磷灰石能够更好的促进细胞的粘附和爬行。结论兼具优异的理化性能和生物活性的Sr HA/PMMA复合骨水泥,有着广阔的骨科微创修复应用前景。Objective The purpose of this study is to harness theadvantages of both PMMA and Sr HA in order to prepare a new kind of composite bone cement（Sr HA/PMMA） with goodmechanical strength,improved exothermic effect and osteogenic activity.The effect of Sr HAon the in vitrosetting behaviors,and mechanical and biological properties of composite cements wassystematically investigated.Methods Hydrothermal synthesis of Sr HA was introduced into PMMA to prepare a novel Sr HA/PMMA composite bone cement,and the mechanical property,setting time,heat release and the bioactivity was systematically investigated.After that,cytotoxicity,cell adhesion and proliferation of Sr HA/PMMA composite bone cement was evaluated by cocultured the cell with materials.Results The results showed that the prepared Sr HA/PMMA composite bone cementsrevealed significantly decreased polymerization temperature as compared with pure PMMA,butmaintained ideal setting times（8 to 11min）and high mechanical strength（90MPa for compressivestrength）.Most interestingly,the incorporation of Sr HAinto PMMA improved its cell/material interaction,asindicated by the significantly enhanced apatite-mineralization ability and stimulatory effect on the attachment and spread of osteoblasts.Conclusion The results suggest that Sr HA/PMMA composite bone cements possess distinctive physicochemical and bioactive properties,and are apromising injectable biomaterial for orthopedic applications.

关 键 词：骨水泥 锶磷灰石 PMMA 凝固时间 生物活性 生物矿化 细胞毒性 粘附 增殖 骨整合性能 

分 类 号：R68[医药卫生—骨科学] R318.08[医药卫生—外科学]