微米级椭球形掺铕羟基磷灰石的发光和细胞毒性研究（英文）  

Luminescent and Cytotoxic Characteristics of an Ellipsoidal and Microsized Europium(Eu)-doped Hydroxyapatite

作　　者：马铭[1] 卢伟鹏[1] 曹霄峰[1] 毛克亚[2] 郭燕川[1] 

机构地区：[1]中国科学院理化技术研究所,光化学转化与功能材料重点实验室,北京100190 [2]解放军总医院(301医院),北京100853

出　　处：《无机材料学报》2016年第8期890-896,共7页Journal of Inorganic Materials

基　　金：Program of International Science and Technology Corporation,China(2013DFA50920);Science and Technology of Beijing,China(Z131100005213007);National Natural Sciences Foundation of China(51372276)

摘　　要：利用碳酸钙模板和离子交换过程成功制备了一种新型掺铕羟基磷灰石微球。通过扫描电子显微镜（SEM）、X射线衍射（XRD）、感应耦合等离子体（ICP）、光致发光谱和体外MTT实验对微球物化性能、发光性和细胞毒性进行了表征。研究结果显示：掺铕羟基磷灰石微球呈椭球形,直径约为2.5μm,长度约为7μm,并具有刺猬状表面;在晶体结构上与纯相六方羟基磷灰石相同,具有高结晶性;铕离子成功进入羟基磷灰石晶相。同时,研究还表明该掺铕羟基磷灰石颗粒在紫外光激发下显示出红色荧光。光致发光光谱表明铕离子的^5D_0→^7F_（1-4）跃迁明显在光致发光活性中具有重要作用。体外细胞毒性实验结果显示：MC3T3-E1小鼠胚胎骨细胞在掺铕羟基磷灰石浸提液中能够持续增殖,没有细胞形态上的变化,并且细胞相对增值率计算结果表明该掺铕羟基磷灰石细胞毒性等级为1级。A novel europium（Eu^（3＋））-doped hydroxyapatite microspheres（Eu-HAp Ms） was successfully prepared via the calcium carbonate template and ion exchange process. The physicochemical, luminescent and cytotoxic properties of the prepared Eu-HAp Ms powders were investigated by the Scanning Electron Microscope（SEM）, X-ray Diffraction（XRD）, Inductively Coupled Plasma（ICP）, and MTT assays in vitro. Results showed that Eu-HAp Ms particles exhibited an ellipsoidal morphology with diameters of about 2.5 μm and lengths of about 7 μm with a hedgehog-like surface. Besides, the pure hexagonal phase of hydroxyapatite with high crystallinity of Eu-HAp Ms and successful doping of Eu^（3＋） ions into HAp Ms were revealed. The present study showed that the Eu-HAp Ms powders actually displayed red fluorescence under ultraviolet（UV） irradiation. It was evident that the ^5D_0→^7F_（1-4）transitions of Eu（III） ions played a key role in the photoluminescence（PL） activity. Finally, the MTT assay results revealed the continuous proliferation and no morphological change of MC3T3-E1 cells co-cultured with the extracts of Eu-HAp Ms powders and the calculated relative growth rate（RGR） indicating the cytotoxicity grade at level 1.

关 键 词：羟基磷灰石 生物材料 铕掺杂 椭球形 荧光性 

分 类 号：TQ174[化学工程—陶瓷工业]