CCL2-2518位点基因多态性与重症肠道病毒71型感染相关性研究  

Relationship between gene polymorphism of CCL2- 2518 and severity of enterovirus 71 infection

作　　者：贾文芳[1] 刘培培[1] 胡静飞[1] 李菲[1] 吕华[1] 甄媛媛 宋龙[1] 陈宗波[1] 

机构地区：[1]青岛大学附属医院儿科,山东青岛266003

出　　处：《中国实用儿科杂志》2016年第9期686-689,共4页Chinese Journal of Practical Pediatrics

基　　金：国家自然科学基金项目(31171212)

摘　　要：目的研究CCL2-2518位点基因多态性与儿童重症肠道病毒71型(EV71)感染的相关性。方法收集2014年5月至2015年9月在青岛大学附属医院儿科检测为EV71阳性的手足口病合并中枢神经系统感染患儿188例,根据病情轻重分为轻症组和重症组。同时选择正常体检儿童235例作为正常对照组。提取患儿外周血白细胞基因组DNA,用多重高温连接酶技术(i MLDR)检测EV71感染患儿及正常对照组CCL2-2518位点基因多态性,酶联免疫吸附法(ELISA)测定血清CCL2浓度。结果 EV71感染患儿与对照组相比,CCL2-2518位点各基因型分布及等位基因频率间差异无统计学意义(P>0.05)。重症组与轻症组相比,GG基因型和G等位基因频率更高(P<0.001)。EV71感染组血清CCL2浓度明显高于对照组(P<0.05),其中重症组CCL2浓度较轻症组及对照组均明显升高(P<0.05)。EV71感染组CCL2-2518位点各基因型间血清CCL2浓度比较:GG型高于AG型,AG型高于AA型,含G等位基因者(GG+AG)高于非G等位基因携带者(AA)(P<0.05)。结论 CCL2-2518位点G等位基因与EV71感染的严重程度相关,可能是EV71感染的易感因素。Objective To investigate the relationship between CCL2-2518A/G gene polymorphism and severity of enterovirus 71 （EV71） infection. Methods CCL2 gene polymorphism in 188 EV71-infected patients and 235 healthy controls were detected by the improved multiplex ligation detection reaction technique （iMLDR）. The level of CCL2 in two groups was determined by enzyme-linked immunosorbent assay （ELISA）. Results No significant differences were found in the distribution of genotype CCL2-2518A/G between EV71-infected patients and the healthy control group（P 〉 0.05 ）. The G allele （genotypes AG or GG） in the CCL2-2518A/G（P 〈 0.001 ） was more frequent in patients with severe EV71 infection. The level of CCL2 in infected patients was higher than that of the heathy controls （P 〈 0.05 ） ; the severe cases had higher level of C CL2 than that of the slight cases and healthy controls （P 〈 0.05）. The level of CCL2 in GG gene group was significantly higher than that in AG gene group and the level of CCL2 in AG gene group was significantly higher than that in AA gene group. The people with CCL2-2518G allele （GG＋AG） had higher level of CCL2 than those only with CCL2-2518A allele （ AA ） （P 〈 0.05 ）. Conclusion The G carrier of the CCL2-2518MG is found to be associated with severity of EV71 infection, and could be susceptibility factors in the development of EV71 infection.

关 键 词：肠道病毒71 CCL2—2518A/G 单核苷酸 多态性 

分 类 号：R72[医药卫生—儿科]