运用核磁共振技术揭示生物固体修复土壤中胡敏酸的化学结构  

Chemical Structure of Humic Acids in Biosolids-amended Soils as Revealed by NMR Spectroscopy

作　　者：C.Y.Chiu G.Tian 张水勤[3] 袁亮[3] 赵秉强[3] 

机构地区：[1]台湾中央研究院生物多样化研究中心,台北11529 [2]美国芝加哥大都会水再生利用区环境监测研究部监测研究司略兴研发室,西塞罗il60804 [3]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《腐植酸》2016年第4期34-39,共6页Humic Acid

摘　　要：应用核磁共振技术表征长期施用2个水平生物固体的土壤中提取的胡敏酸特征,以评估生物固体修复土壤中有机碳的稳定性。同时,量化土壤中富里酸、胡敏酸和Fe/Al氧化物含量。土壤样品采集于2004年,于伊利诺斯州西南部的富尔顿县选取7个地块进行取样,取样地块生物固体的累积施用量分别为0(对照)、554(低施用量)和1066 mg/hm^2(高施用量)。结果表明,生物固体的施用增加了土壤富里酸与胡敏酸的含量,但是生物固体修复的土壤和对照土壤的FA/HA比值没有差异。此外,生物固体的施用不影响水溶性有机碳的含量,却能增加土壤有机复合体中Fe/Al含量,降低其C/Fe和C/Al比例。^(13)C核磁共振波谱结果表明,随着生物固体的施用土壤胡敏酸中烷基碳增加和芳香碳降低,且施用量越高,变化程度越大;土壤胡敏酸碳结构也从氧烷基主导变为烷基主导。生物固体的施用不能降低土壤有机碳的稳定性,相反,能够增加土壤腐殖质的稳定性。We used NMR spectroscopy to characterize humid acids extracted from soils that had received long-term application of 2 levels of biosolids to evaluate the soil organic matter （SOM） stability in biosolids-amended soils. The study also quantified fulvic acids （FAs）, humic acids （HAs） and Fe/Al oxides. The soils were collected in 2004 from 7 fields, in Fulton County, southwestern Illinois, which received biosolids at a cumulative rate of 0 （control）, 554 mg/hm^2 （low biosolids） and 1066 mg/hm^2 （high biosolids）. The application of biosolids increased both FA and HA contents, but biosolids-amended soil and control soil did not differ in FA/HA ratio. Biosolids application had no effect on water-soluble organic carbon content Biosolids application increased the presence of Fe/Al in the SOM complex and lowered its C/Fe and C/A1 ratios. 13C NMR spectra showed increased alkyl C and decreased aromatic C content in soil HAs with the application of biosolids, and the extent of such changes was higher with high than low biosolids treatment. Under biosolids application, the soil HAs＇ C structure shifts from O-alkyl-dominant to alkyl-dominant. Biosolids application does not decrease SOM stability but rather increases the stability of soil humic substances.

关 键 词：非晶型Fe/Al氧化物 生物固体 碳化学结构 胡敏酸 美国中西部 核磁共振 土壤有机碳稳定性 

分 类 号：S153[农业科学—土壤学]