普通野生稻转育后代稻褐飞虱抗性基因的初步定位  

Preliminarily mapping quantitative trait loci( QTLs) for brown planthopper resistance in the offspring of Oryza rufipogin Griff

作　　者：张志伟[1] 段利超 黄常源 林拥军[1] 陈浩[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室/国家植物基因研究中心(武汉),武汉430070

出　　处：《华中农业大学学报》2016年第2期10-14,共5页Journal of Huazhong Agricultural University

基　　金：国家"863"计划项目(2014AA10A604)

摘　　要：通过BR96与白56构建F2群体,利用均匀分布于水稻全基因组的122对多态性引物标记分析F2群体的基因型,构建遗传连锁图谱,考查F2衍生的F3各家系的褐飞虱苗期抗性等级,检测水稻褐飞虱抗性数量性状位点(QTL)。结果显示:分别在第3、4和6号染色体上各扫描到一个抗褐飞虱QTL位点,QBph3位于第3染色体RM489-RM282之间,LOD值为5.1,解释表型变异率是3.8%;QBph4位于第4号染色体RM16605-RM16717之间,LOD值为28.7,解释表型变异率是29.4%;QBph6位于第6号染色体RM276-RM527之间,LOD值为2.7,解释表型变异率是7.1%;3个QTL的联合贡献率为40.3%。Qbph4可解释表型变异率最大,初步判断Qbph4可能是一个控制褐飞虱抗性的主效基因。The genetic linkage map was constructed by using one F2：3 population of crossing BR96 with Bai56 and 122 molecular makers covering the whole rice genome. The quantitative trait loci （QTLs） for BPH resistance were identified based on marker geneotypes and the pheontypes of BPH resistance in rice seedlings. Totally three QTLs for BPH resistance were detected. QBph3 was detected in the long arm of chromosome 3 between the molecular markers RM489 and RM282,with a LOD value of 5.1 and explained 3.8% the phenotypic variation. QBph4 was detected in the chromosome 4 between the molecu- lar markers RM16605 and RM16717,with a LOD value of 28.7 and explained 29.4% the phenotypic var iation. QBpk6 was detected in the short arm of chromosome 6 between the molecular markers RM276 and RM527,with a LOD value of 2.7 and explained 7. 1% the phenotypic variation. Obviously, Q13ph4 with the largest effect among three identified QTLs may be a major QTL for BPH.

关 键 词：普通野生稻 重组自交系 褐飞虱 QTL 遗传连锁图谱 基因定位 

分 类 号：S511.103.53[农业科学—作物学]