1株内生真菌的分离鉴定及其对铁皮石斛的促生作用  

Isolation and identification of endophytic fungus Devriesia lagerstroem and its promoting effect on Dendrobium candidum growth

作　　者：谢玲[1,2,3] 张雯龙[2] 蓝桃菊[2] 陈艳露[2] 覃丽萍[2] NARISAWA Kazuhiko 刘斌[1] 

机构地区：[1]广西大学农学院应用微生物研究所,南宁530005 [2]广西农业科学院微生物研究所,南宁530007 [3]日本茨城大学农学院

出　　处：《华中农业大学学报》2016年第3期83-88,共6页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(31460016);广西自然科学基金项目(2014GXNSFBA118101;2015GXNSFBA139083);广西农业科学院基本科研业务专项(2015YT80);广西农业科学院科技发展基金项目(2015JZ14;2015YM04)

摘　　要：从不同植物的茎和叶中分离获得204株真菌,通过对白菜的致病性测定筛选获得1株能显著促进白菜生长的内生真菌菌株24L-4。结合形态学特征观察进行ITS-r DNA和18S-r DNA序列分析,对其进行鉴定,确定为Devriesia lagerstroem。该菌株对铁皮石斛组培苗的株高、叶宽、茎径和干质量分别较未接种对照处理增加33.3%、65.7%、27.3%和45.1%,显著高于对照处理。同时,盆栽试验表明:D.lagerstroem铁皮石斛盆栽苗的茎径、鲜质量和干质量分别较无接种对照增加104.0%、83.9%和114.7%,显著高于对照处理。显微镜观察发现:D.lagerstroem可定殖于铁皮石斛根部的外皮层和皮层。研究结果表明,D.lagerstroem对铁皮石斛具有明显的促生作用,可用于铁皮石斛菌肥的开发。Two hundred and four fungal isolates were obtained from stems and leaves of different plants.One endophytic fungal isolate 24L-4 was selected by pathogenicity test on Chinese cabbage.Isolate 24L-4 was identified as Devriesia lagerstroem based on its morphological characteristics and sequences of internal transcribed spacer and 18S ribosomal DNA.Isolate 24L-4 was inoculated on Dendrobium candi- dum tissue cultures and pot seedlings,respectively,with non-inoculated seedlings as the control.The re- suits showed that the seedling height, leaf width, stem diameter and dry weigh were significantly en- hanced by 33.3% ,65.7% ,27.3% and 45.1% ,respectively,in the tissue cultures.Similar results were ob- served in the pot experiment with increase percentages by 104.0%, 83.9% and 114.7% for the stem di- ameter, fresh weight and dry weight, respectively.The hyphae and microseierotia-like structures of isolate 24L4 were found in the root epidermal and cortex of D. candidum.These results indicated that isolate 24L-4 had excellent growth promoting effect on D. candidum.

关 键 词：Devriesia lagerstroem 铁皮石斛 内生真菌 促生 

分 类 号：Q939.9[生物学—微生物学]