东澳棕叶片和根系对干旱和复水的生理响应  

Physiological Changes in the Leaves and Roots of Carpentaria acuminata to Drought Stress and Re-watering Process

作　　者：阮志平[1] 

机构地区：[1]厦门市园林植物园,福建厦门361003

出　　处：《西北林学院学报》2016年第5期90-95,245,共7页Journal of Northwest Forestry University

基　　金：厦门市科技计划项目(3502Z20144071)

摘　　要：为了研究植物整体对干旱胁迫和复水的生理响应,以2年生的东澳棕(Carpentaria acuminata)实生苗为材料,分别进行干旱胁迫0、7、14d和21d,再复水3d和6d,测定叶片和根系的超氧化物歧化酶(SOD)活性、脯氨酸(Pro)含量、可溶性蛋白含量、丙二醛(MDA)含量、相对电导率和叶绿素含量的生理指标,用于明确东澳棕叶片和根系对对干旱胁迫和复水的生理响应。结果表明:叶片的SOD活性、Pro含量、可溶性蛋白含量、MDA含量在轻度干旱胁迫7d时显著增加,随着干旱进行而减少,复水后又增加,相对电导率的变化规律与之相反。叶绿素含量在干旱胁迫21d时达到最大值,复水后降低。根系的SOD活性只在干旱胁迫21d时显著提高,复水后显著降低;Pro含量在干旱胁迫14d时升高,后降低,复水后升高;可溶性蛋白含量在干旱胁迫7d时升高,后降低,复水后升高,MDA含量干旱胁迫7d时降低,干旱胁迫21d时升高,复水后降低,根系的相对电导率在干旱胁迫7d时略增加,后降低。叶片的SOD活性和MDA含量在干旱7d-复水期间的变化趋势相同,根系的SOD活性和MDA含量在干旱7d-复水期间的变化趋势也相同,说明叶片和根系之间、SOD和MDA之间具有协同效应。干旱7d-复水期间叶片的SOD活性和MDA含量显著高于根系,干旱14d-复水期间叶片的Pro含量显著低于根系。植物地上部和根系之间存在协同和互补效应,有利于提高植物的水分的适应性。To evaluate the physiological responses of Carpentaria acurninata to drought stress and re-watering process,the activities of superoxide dismutase （SOD） and the contents of malondialdehyde（MDA）, proline, soluble protein, relative electrical conductivity, chlorophyll a, b and chlorophyll （a＋b） in the leaves and roots of C. acurninata were determined when the two-year seedlings were subjected to 0 d（control）, 7 d, 14 d,21 d drought stresses,and 3 d,6 d re-watering. The results showed the activities of SOD,the con- tents of proline,the contents of soluble protein and the contents of MDA in the leaves increased significant- ly under 7 d drought stress, then decreased,which were contrary to the pattern for the relative conductivity in the leaves. The contents of chlorophyll could reach to the maximum value under 21 d drought stress then dropped steeply after re-watering. The activities of SOD in the roots increased significantly under 21 d drought stress and decreased significantly under re-watering. The contents of proline in the roots increased under 14 d drought stress,then decreased,and improved when re-watering. The contents of soluble protein in the roots increased under 7 d drought stress, then decreased,and improved when re-watering. The con- tents of MDA in the roots decreased under 7 d drought stress ,then significantly increased under 21 d drought stress and decreased under re-watering. The relative conductivity in the roots increased slightly under 7 d drought stress, and then decreased. The activities of SOD and the contents of MDA in the leaves had the same dynamic tendency from 7d drought stress to re-watering. The same change trend was observed in the roots for the activities of SOD and the contents of MDA from 7 d drought stress to re-watering. The activities of SOD and the contents of MDA in the leaves were significantly higher than those in the roots. However,the contents of proline in the leaves were drastically lower than those in the roots. The synergistic and compensation effec

关 键 词：东澳棕 干旱胁迫 复水 叶片 根系 生理响应 

分 类 号：S718.3[农业科学—林学]