10株白僵菌菌株ITS序列测定和系统发育分析  

Internal Transcribed Spacer Region Sequencing and Phylogenetic Analysis of 10 Strains of Beauveria spp.

作　　者：蔡三山[1] 欧阳静 王义勋[1] 查玉平[1] 洪承昊[1] 陈京元[1] 

机构地区：[1]湖北省林业科学研究院,湖北武汉430075 [2]湖北省公安县植物保护局,湖北公安434300

出　　处：《西北林学院学报》2016年第5期182-187,共6页Journal of Northwest Forestry University

基　　金：林业公益性行业科研专项经费项目(201404409)

摘　　要：通过对10株白僵菌菌株进行培养和提取基因组DNA,并利用真菌ITS序列通用引物ITS4和ITS5进行PCR扩增,均得到了1条长约500bp DNA产物。分别对该产物进行序列测定和分析表明,这10株白僵菌菌株的ITS序列长为490~494bp;利用ITS序列构建系统发育树表明,菌株YD-1、YD-2、JN-1、QC-1、SY-1、YC-1、YC-2、GA-1和FJ-2均为球孢白僵菌（Beauveria bassania）,而菌株FJ-1很可能为白僵菌属的一个新成员。Based on cultivation and extracting genomic DNA of 10 strains of Beauveria spp. ,we amplified a DNA fragment with a length of about 500 bp and the fungal universal primers,ITS4 and ITS5. Sequence analysis showed that the length of ITS region of 10 strains was from 490 bp to 494 bp. Phylogenetic analysis demonstrated that the 9 strains, including YD-1, YD-2, JN-1, QC-1, SY-1, YC-1, YC-2, GA-1, and FJ-2 be- longed to Beauveria bassania and the left strain FJ-1 maybe a new member of genus Beauveria.

关 键 词：白僵菌 ITS序列 系统发育 

分 类 号：S763.15[农业科学—森林保护学]