干细胞移植治疗噪声性和药物性聋的比较实验研究  

A Comparative and Experimental Study on the Treatment of Noise Induced Hearing Loss and Toxic Hearing Loss with the Stem Cells Transplantation

作　　者：朱汉卿[1] 张军峰[2] 徐侦[3] 

机构地区：[1]南京中医药大学解剖与组胚教研室,南京210029 [2]南京中医药大学微生物与免疫学教研室 [3]南京医科大学第二附属医院

出　　处：《听力学及言语疾病杂志》2016年第5期478-481,共4页Journal of Audiology and Speech Pathology

基　　金：江苏省高校自然科学基金项目(12KJD360002);江苏省高校优势学科建设工程(PAPD)联合资助

摘　　要：目的比较耳蜗干细胞移植治疗噪声性聋豚鼠和药物性聋豚鼠模型的效果。方法选用清洁级健康成年纯白红目豚鼠120只,建立噪声性聋模型耳蜗干细胞移植组、噪声性聋模型空白对照组、药物性聋模型耳蜗干细胞移植组、药物性聋模型空白对照组,每组30只;向噪声性聋模型耳蜗干细胞移植组和药物性聋模型耳蜗干细胞移植组耳蜗分别注入耳蜗干细胞悬液10μl,向噪声性聋模型空白对照组和药物性聋模型空白对照组耳蜗分别注入10μl生理盐水。于术后7、28、56d进行ABR检测并进行免疫荧光观测Nestin阳性细胞数和MyosinⅦA阳性细胞数。结果在噪声性聋和药物性聋耳蜗干细胞移植组的耳蜗鼓阶处观测到Nestin阳性细胞和MyosinⅦA阳性细胞,但噪声性聋干细胞移植组的Nestin阳性细胞和MyosinⅦA阳性细胞数量多于药物性聋干细胞移植组;耳蜗干细胞移植后噪声性聋和药物性聋组ABR反应阈均下降,但术后28天和56天噪声性聋干细胞移植组ABR阈值分别为52.61±5.14和40.39±4.59dB SPL,低于药物性聋干细胞移植组(分别为66.39±4.77和60.41±4.17dB SPL)。结论耳蜗干细胞移植对于噪声性聋豚鼠模型的治疗效果优于药物性聋豚鼠模型。Objective To compare the effect of the cochlear stem cells transplantation in treating the noise in duced hearing loss model and toxic hearing loss model. Methods The Experimental guinea pigs were divided into three groups： the group of the noise induced hearing loss model treated with the cochlear stem cells, the blank con trol group of toxic hearing loss model treated with the cochlear stem cells and noise induced hearing loss model and the blank control group of toxic hearing loss model. The cochlear stem cells suspension and physiological saline were transplanted into the cochlear inner ears of the three groups, respectively. ABR detection and immunofluorescence observation were implemented three times in 7 days, 28days and 56 days a{ter the operation, respectively. Results Nestin positive cells and Myosin ~ A positive cells were detected in both of the two models treated with cochlear stem cells, but the number of Nestin positive cells and Myosin VII A positive cells detected in noise induced hearing loss model was higher than that in the toxic hearing loss model. The ABR responses decreased on the noise induced hearing loss model and toxic hearing loss model treated with cochlear stem cells transplantation, but the hearing re covery of guinea pigs in the noise induced hearing loss model was superior to the toxic hearing loss model at 28 days and 56 days after operation. Conclusion The cochlear stem cells transplantation is more effective in treating noise in- duced hearing loss than toxic hearing loss model.

关 键 词：耳蜗干细胞 移植 噪声性聋 药物性聋 

分 类 号：R764.433[医药卫生—耳鼻咽喉科]