响应曲面法优化Gluconobacter melanlgenu X42培养基  

Gluconobacter melanogenus X42 Medium Optimized by Response Curved Surface Methodology

作　　者：陈羽[1] 徐威[1] 位星[1] 胡思宇[1] 

机构地区：[1]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《微生物学杂志》2016年第4期67-70,共4页Journal of Microbiology

基　　金：辽宁省教育厅一般项目(L2013380);辽宁省教育厅高等学校创新团队项目(LT2014023)

摘　　要：采用Plackett-Burman设计、最陡爬坡试验和中心组合试验相结合的策略对Gluconobacter melanlgenu X42转化培养基进行优化。试验结果表明:最佳转化培养基组成为麦芽抽提物0.46 g/L、蛋白胨2.07 g/L、Ca CO30.70 g/L、Zn SO40.04 g/L。优化后L-山梨糖生成量提高了9.9%。可以应用响应曲面法对G.melanlgenu X42转化培养基进行优化。The transformation medium of Gluconobacter melanogenus X42 was optimized with the tactics of PlackettBurman design,slope-climbing test and central combination test.The experimental results showed that the optimum transformation medium components were： malt extract 0.46 g / L,peptone 2.07 g / L,Ca CO30.70 g / L and Zn SO40.04 g / L.L-sorbose production was increased by 9.9% after the optimization.G.melanogenus X42 transformation medium could be optimized by the response curved surface methodology.

关 键 词：生黑葡萄糖酸杆菌 培养基 响应曲面法 L-山梨糖 条件优化 

分 类 号：Q815[生物学—生物工程]