氯胺酮对发育期海马神经元促炎性因子白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α表达的影响  

Effects of ketamine on expression of pro-inflammatory cytokines interleukin-1β mRNA, interleukin-6 mRNA and tumor necrosis factor-α of rat developing hippocampal neurons in vivo

作　　者：张雪[1] 赵以林[1] 冯丽[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉科,武汉430030

出　　处：《中华实验外科杂志》2016年第9期2114-2116,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察不同剂量不同时间暴露下氯胺酮对发育期海马神经元促炎性因子白细胞介素（IL）-1β、IL-6和肿瘤坏死因子-α （TNF-α ）表达的影响.方法 新生鼠龄5d SD大鼠80只,为研究剂量依赖性,随机分为氯胺酮0.5mg、1.0mg、2.0mg、10.0 mg组和对照组（C组）.为研究时间依赖性,随机分为（2.0 mg/kg）氯胺酮注射后2h组、4h组、6h组和24 h氯胺酮组和对照组（C组）.氯胺酮组大鼠皮下注射氯胺酮干预.对照组注射0.9％生理盐水.干预后在各对应时间点取海马神经元提取RNA,利用实时定量聚合酶链反应（Real-time PCR）进行定量分析促炎性因子IL-1β、IL-6和TNF-α mRNA表达水平.结果 与C组比较,1 mg/kg氯胺酮注射后明显抑制IL-1β、IL-6和TNF-α mRNA表达,呈剂量依赖性.用（2.0 mg/kg）氯胺酮处理后,海马神经元IL-1β mRNA表达在4h开始下调,6h达到最低（0.66±0.15,P＜0.05）,而IL-6和TNF-α mRNA表达在2h开始下调,4h达到最低（0.73 ±0.06,0.72±0.09,P＜0.05）.麻醉停止24 h后IL-1β、IL-6和TNF-α mRNA表达恢复正常.结论 用氯胺酮处理后剂量时间依赖性短暂下调海马神经元促炎性因子IL-1β、IL-6和TNF-α 表达。Objective To investigate the dose-and time-dependent effects of ketamine on expression of interleukin （IL）-1β mRNA,IL-6 mRNA and tumor necrosis factor （TNF）-α of rat developing hippocampal neurons in vivo.Methods Eighty 5-day-old SD rats were randomly divided into groups：to investigate the dose-dependent effect of ketamine,treatment groups which received 0.5,1.0,2.0,10.0 mg/kg ketamine injection and control group received 0.9% saline treatment;to investigate the time-dependent effect of ketamine,treatment groups which received 2.0 mg/kg ketamine injection for 2,4,6 and 24 h and control group received 0.9% saline treatment.Total RNA was extracted from Pups hippocampi killed by decapitation after intervention.Real-time PCR was used to detect the expressions of the IL-1β mRNA,IL-6 mRNA and TNF-α mRNA after treatment.Results Ketamine （1.0,2.0,10.0 mg/kg） dose-dependently decreased the expressions of the IL-1 β mRNA,IL-6 mRNA and TNF-α mRNA （0.66 ± 0.15,P 〈 0.05）.And compared with the group C,there are significant decrease in the expressions of IL-1β mRNA at 4-6 h,IL-6 mRNA at 2-6 h and TNF-o mRNA at 2-6 h after 2.0 mg/kg ketamine injection （0.73 ± 0.06,0.72 ± 0.09,P 〈 0.05）.Conclusion There are significant dose-and time-dependent decrease in the expressions of IL-1β,IL-6 and TNF-α after ketamine intervention in the developing hippocampal neurons.

关 键 词：氯胺酮 海马 肿瘤坏死因子-Α 白细胞介素 

分 类 号：R392.12[医药卫生—免疫学]