^(56)Fe^(17+)重离子束对人淋巴细胞磷酸化组蛋白H2AX表达影响研究  

Effect of ^(56)Fe^(17+) heavy ion on the expression of phosphorylated histone H2AX of human lymphocytes

作　　者：董娟聪[1] 原雅艺 张睿凤 党旭红[1] 张忠新[1] 刘建功[1] 左雅慧[1] 段志凯[1] 

机构地区：[1]中国辐射防护研究院,山西太原030006

出　　处：《中国职业医学》2016年第4期471-474,共4页China Occupational Medicine

摘　　要：目的探讨^(56)Fe^(17+)重离子束对人淋巴细胞磷酸化组蛋白H2AX(γH2AX)表达的影响。方法取非洲淋巴细胞瘤病毒转化正常人B淋巴细胞(Peng-EBV细胞),分别予照射剂量为0.0(对照组)、0.1、0.3、0.5、0.7、1.0、2.0Gy的^(56)Fe^(17+)重离子照射,照射剂量率为0.23~0.55 Gy/min,分别于照射后0、2、4、8、48及72 h采用流式细胞术检测细胞γH2AX的表达量。结果γH2AX表达水平在56Fe12+重离子照射剂量与照射后处理时间之间存在交互效应(P<0.01)。照射后2~72 h,γH2AX的表达水平在照射剂量为0.3~2.0 Gy时均高于同时间点对照组(P<0.05)。在0.3~2.0 Gy范围内,γH2AX的表达水平在照射后8~72 h均低于同组照射后2和4 h时间点的表达水平(P<0.05)。当照射剂量为0.5、1.0或2.0 Gy时,γH2AX的表达水平照射后72 h内呈随着照射后时间的延长而下降的趋势(P<0.05)。在照射后2~4 h,当照射剂量为0.0~1.0 Gy时,γH2AX表达水平随照射剂量增加而增加(P<0.01)。结论在照射剂量为0.0~1.0时,^(56)Fe^(17+)重离子诱导的Peng-EBV细胞γH2AX表达水平于照射后2~4 h内存在剂量-响应关系。Objective To explore the effect of ^56Fe^17＋ heavy ion on the expression of phosphorylated histone H2AX（ γH2AX） of human lymphccytes. Methods The Epstein-Barr virus transformed human B lymphocyte cell lines（ PengEBV） were selected and exposed to^56Fe^17＋heavy ion at irradiation dose of 0. 0（ control group）,0. 1,0. 3,0. 5,0. 7,1. 0 and 2. 0 Gy,respectively,with the dosing rate of 0. 23-0. 55 Gy / min. Flow-cytometry was used to detect the changes of expression of γH2AX at time points of 0,2,4,8,48 and 72 hours after irradiation. Results The expression of γH2AX showed interaction existed between radiation dose and the treatment time after radiation（ P〈0. 01）. Compared with the control at the same time points,the expression of γH2AX increased at the dose of 0. 3-2. 0 Gy and the time points of 2-72hours（ P〈0. 05）. The expression of γH2AX at the dose of 0. 3-2. 0 Gy and time points of 8-72 hours was lower than those at the same dose and time points of 2 and 4 hours（ P〈0. 05）. When the dose was at 0. 5,1. 0 or 2. 0 Gy,the expression of γH2AX decreased with the increasing time of exposure in 72 hours（ P〈0. 05）. At the dose of 0. 0-1. 0 Gy and the time points of 2-4 hours,the expression of γH2AX increased with the increasing dose of irradiation（ P〈0. 01）. Conclusion The expression of γH2AX in Peng-EBV cells shows a dose-response relationship within 2-4 hours after 0. 0-1. 0 Gy irradiation of^56Fe^17＋.

关 键 词：^56Fe^17+重离子 DNA损伤修复 淋巴细胞 磷酸化组蛋白H2AX 

分 类 号：R14[医药卫生—公共卫生与预防医学] X591[环境科学与工程—环境工程]