地西他滨联合紫杉醇对耐药MCF-7细胞增殖抑制和诱导凋亡的增敏作用  

Sensitization of Decitabine Combined with Paclitaxel on Proliferation Inhibition and Apoptosis Induction of Paclitaxel-resistant MCF-7 Cells

作　　者：张敏[1] 沈杰[1] 周海峰[1] 高宁舟[1] 张云轩[1] 傅峰[1] 宋钟娟[1] 

机构地区：[1]复旦大学附属华东医院药剂科,上海200040

出　　处：《中国药师》2016年第9期1638-1640,共3页China Pharmacist

摘　　要：目的:本研究旨在探讨地西他滨联合紫杉醇对紫杉醇耐药细胞MCF-7的细胞增殖抑制和诱导凋亡作用的影响。方法:采用CCK-8方法检测联合用药(地西他滨+紫杉醇)或单独用药(地西他滨、紫杉醇)组不同给药浓度在24,48,72 h对耐药细胞的增殖抑制作用,流式细胞仪检测不同给药组给药后48 h的细胞凋亡率。结果:CCK-8结果显示联合用药组相较于单药组对细胞增殖抑制作用显著增加(P<0.05),呈剂量依赖性,不同给药组的细胞增殖抑制率随着时间延长而增大;诱导细胞凋亡结果显示,48 h地西他滨单药组和紫杉醇单药组的细胞凋亡率分别是(20.4±1.98)%和(21.8±3.34)%,联合用药组的细胞凋亡率增加至(70.8±8.23)%。结论:地西他滨联合紫杉醇对紫杉醇耐药MCF-7细胞株的增殖抑制作用增加,促进耐药细胞的凋亡,地西他滨联合紫杉醇对耐药细胞株具有协同抗肿瘤作用。Objective： To explore the anti-proliferation and apoptosis-inducing effects of decitabine combined with paclitaxeI on paelitaxel-resistant MCF-7 cells. Methods： Cell counting kit-8 （ CCK-8 ） assays were used to determine the proliferation inhibition of drugs at different concentrations in 24, 48 and 72h. The group was treated with decitabine, paelitaxel and the combination of the two drugs, respectively. The cell apoptosis rate was determined by flow eytometry after the treatment with the drugs at different concentrations in 48h. Results： The results of CCK-8 showed the combination group significantly inhibited the cell proliferation when compared with the single drug use group（P 〈 0.05 ） , and the anti-proliferation value was in a dose-dependent manner in all groups. The apoptosis values after the treatment with decitabine, paclitaxel and the combination in 48h was （20.4 ± 1.98 ）% , （21.8± 3.34 ）% and （70.8 ± 8.23 ） %, respectively. Conclusion： The proliferation inhibition and apoptosis induction are both increased significantly in the combination group. Synergistic effect of decitabine and paclitaxel is found in muhidrug resistant breast cancer cell line MCF-7 in vitro.

关 键 词：地西他滨 紫杉醇耐药 MCF-7细胞株 细胞增殖抑制 细胞凋亡 

分 类 号：R965.1[医药卫生—药理学]