川牛膝耐寒性筛选及其耐寒生理特性的研究  

Screening and Research Cyathula officinalis in Cold Hardy Physiological Characteristics

作　　者：颜旭[1,2] 杨世民[1] 马云桐[3] 陈雅歆 谭友莉[3] 吴翠平[1] 

机构地区：[1]四川农业大学农学院,四川成都611130 [2]成都市农林科学院,四川成都611130 [3]成都中医药大学药学院,四川成都611137

出　　处：《中药材》2016年第5期962-966,共5页Journal of Chinese Medicinal Materials

基　　金：四川省科技厅科技支撑计划(2011SZ0282)

摘　　要：目的:探索川牛膝幼苗抗寒机理,为川牛膝品种选育和抗逆栽培提供理论基础。方法:以川牛膝主产区收集的川牛膝种子生长至5对生叶的幼苗为试验材料,通过低温胁迫,测定形态指标和生理指标,用隶属函数法和低温变异指数对川牛膝进行耐寒性筛选与评价。结果:可溶性糖、脯氨酸、可溶性蛋白、鲜重、株高可作为川牛膝幼苗耐寒性的评价指标;宝兴县崇兴村的红牛膝、宝兴县崇兴村的白牛膝、汉源县的白牛膝耐寒性较强。结论:不同产地川牛膝耐寒性不同,耐寒性与川牛膝幼苗体内的渗透调节物质成正相关。Objective： To explore the mechanism in cold-resistant of Cyathula officinalis seedlings,in order to provide a theoretical basis for variety breeding and resistance cultivation of Cyathula officinalis. Methods： Collected the materials in main producing areas of Cyathula officinalis,and the Cyathula officinalis growing into seedling with five pairs of leaves were used to research. The seeds were deal with low temperature stress,and the seedlings of morphological and physiological indicators of Cyathula officinalis were measured,membership function method and low temperature variation index were used to screen and evaluate the cold-tolerance of the Cyathula officinalis. Results： Soluble sugar,proline,soluble protein,fresh weight,plant height can be used as the evaluation indexes of cold-resistant Cyathula officinalis. The red and white Cyathula officinalis from Baoxing county in Chongxing village and the white Cyathula officinalis from Hanyuan county all had a good cold-resistance. Conclusion： The cold-resistant in Cyathula officinalis are different in different areas,and cold tolerance is positively correlated with the content of osmotic adjustment substance of Cyathula officinalis seedlings.

关 键 词：川牛膝 低温胁迫 筛选 渗透调节物质 

分 类 号：R282.2[医药卫生—中药学]