UHPLC测定不同产地芍药根的有效成分  

Determination of Bioactive Components in Paeonia lactiflora Roots Cultivated in Various Areas by UHPLC

作　　者：刘永强[1] 马越[1] 杨博文[1] 肖佳佳[1] 龙飞[1,2] 许志刚 吕光华[1] 

机构地区：[1]成都中医药大学药学院/中药资源系统研究与开发利用国家重点实验室培育基地/中药材标准化教育部重点实验室,四川成都611137 [2]中江县万生农业科技有限责任公司,四川德阳618100

出　　处：《中药材》2016年第5期980-985,共6页Journal of Chinese Medicinal Materials

基　　金：四川省科技支撑计划项目(2015SZ0040);四川省德阳市"双百工程"创新人才项目(2014年)

摘　　要：目的：以芍药苷等多种有效成分为指标,评价不同产地芍药根的质量。方法：从四川、浙江和安徽三大芍药（白芍）主产区采集了66份芍药根样品,应用UHPLC-DAD技术测定芍药苷、氧化芍药苷、芍药内酯苷、1,2,3,4,6-五没食子酰葡萄糖、苯甲酰芍药苷、丹皮酚、没食子酸、儿茶素的含量,并对各样品中的有效成分进行主成分分析。结果：芍药根中这8种有效成分在30 min内分离良好,峰面积与进样浓度呈良好的线性关系（r≥0.9990,n=6）,加样回收率为95.94%~100.92%（n=6）。四川、浙江、安徽产的芍药根中芍药苷的含量分别为40.54 mg/g（n=23）、33.09 mg/g（n=22）、39.47 mg/g（n=21）。三大芍药产地的样品中这8种有效成分经主成分分析的综合得分为0.4435（n=23,四川）、0.0122（n=22,浙江）和-4.9850（n=21,安徽）。2年生、3年生、4年生、5年生和6年生芍药根中芍药苷的含量分别为24.76（n=2）、37.17（n=16）、37.83（n=23）、39.71（n=16）和37.45 mg/g（n=7）。结论：建立的方法能准确测定芍药根中8种有效成分的含量;以芍药苷或多种有效成分综合得分为评价指标,四川、浙江和安徽种植芍药根的质量不同,以四川产的芍药根最优;根据产量和质量综合分析,芍药根的采收以3~4年生为宜。Objective： To assess the quality of Paeonia lactiflora roots by the multi-bioactive chemical markers. Methods： Total of66 Paeonia lactiflora roots samples were collected from Sichuan,Zhejiang and Anhui. An UHPLC-DAD technique was employed to quantify the contents of paeoniflorin,hydroypaeoniflorin,abiflorin,pentagalloyglucose,benzoypaeoni-florin,paeonol,gallic and catechin in these samples. These chemical components in each sample were also calculated by Principal Component Analysis（ PCA）. Results：The eight bioactive components were good separated in 30 min on the UHPLC chromatogram. The correlation coefficients between peak areas and concentration for these bioactive components were not less than 0. 9990（ n = 6）. And their recoveries were in the range of95. 94% ~ 100. 92%（ n = 6）. The contents of paeoniflorin in Paeonia lactiflora roots samples collected from Sichuan,Zhejiang and Anhui were 40. 54 mg / g（ n = 23）,33. 09 mg / g（ n = 22） and 39. 47 mg / g（ n = 21）,respectively. The values of PCA were 0. 4435（ n = 23）for the samples from Sichuan,0. 0122（ n = 22） for the samples from Zhejiang and- 4. 9850（ n = 21） for the samples from Anhui. The content of paeoniflorin in biennial,triennial,four-year,five-year and six-year old Paeonia lactiflora roots were 24. 76（ n = 2）,37. 17（ n= 16）,37. 83（ n = 23）,39. 71（ n = 16） and 37. 45 mg / g（ n = 7）,respectively. Conclusion： The developed method can accurately quantify the content of principal bioactive compounds in Paeonia lactiflora roots. The quality is various among Paeonia lactiflora roots cultivated in Sichuan,Zhejiang and Anhui on the basis of paeoniflorin content or the value of PCA,but the quality of Paeonia lactiflora roots cultivated in Sichuan is the best. Moreover,it is suggested Paeonia lactiflora roots should be harvested in third or fourth year based on the output and quality.

关 键 词：芍药 UHPLC 芍药苷 芍药内酯苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]