牡丹皮炭不同炮制程度的多成分含量变化研究  

Multicomponent Content Difference Among Different Processing Degrees of Moutan Cortex Charcoal

作　　者：黄绮韵 周苏娟[1] 陈求芳[1] 孟江[1] 王淑美[1] 

机构地区：[1]广东药科大学,广东广州510006

出　　处：《中药材》2016年第5期1024-1027,共4页Journal of Chinese Medicinal Materials

基　　金：国家中医药行业科研专项-全国中药饮片炮制技术规范研究(201207004);国家自然科学基金(81102809)

摘　　要：建立HPLC法测定牡丹皮炭不同炮制程度样品中没食子酸、5-羟甲基糠醛、儿茶素和丹皮酚的含量,并比较其含量变化。方法:采用HPLC法,UltimateTMXB-C18色谱柱,以乙腈-0.2%甲酸为流动相梯度洗脱,流速为1.0m L/min,检测波长245 nm,柱温30℃;并采用PCA法对数据进行分析。结果:没食子酸和5-羟甲基糠醛的含量随炮制时间延长而增加,但一定程度后又开始下降;儿茶素和丹皮酚的含量则随着炮制程度的加深而下降。结论:牡丹皮炭不同炮制程度成分含量差异较大,该文可为建立牡丹皮炭质量标准提供科学依据。Objective： To establish a method of simultaneously determining gallic acid,5-HMF,catechin and paeonol in the different processing degrees of Moutan Cortex charcoal by HPLC,and to compare the difference. Methods： The HPLC separation was carried out on a UltimateTMXB-C18column（ 250 mm × 4. 6 mm,5 μm） with a mobile phase of acetonitrile and water containing 0. 5% formic acid. The flow rate was 1. 0 m L / min at 30 ℃ column temperature,and the detection wavelength was 245 nm. Results： The contents of gallic acid and 5-HMF were increased with the extension of processing time,but declined when it reached to a certain extent. And the contents of catechin and paeonol were decreased in the heating process. Conclusion： The differences of compounds content in different processing degree of Moutan Cortex charcoal were greatly. It provides a scientific basis for quality standard of Moutan Cortex charcoal.

关 键 词：炮制程度 牡丹皮炭 HPLC含量测定 主成分分析 

分 类 号：R283.1[医药卫生—中药学]