复方贞术调脂方改善内质网应激防治小鼠NAFLD研究  

Protection of Fufang Zhenzhu Tiaozhi Formula on NAFLD in Mice by Regulating ERS

作　　者：陈智权 叶碧娴 荣向路[2,3] 郭姣[2,3] 

机构地区：[1]广州中医药大学,广东广州510405 [2]广东药科大学,广东广州510006 [3]广东省代谢病中西医结合研究中心,广东广州510006

出　　处：《中药材》2016年第5期1139-1142,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81202619);广东省自然科学基金团队项目(10351022401000000)

摘　　要：目的:观察复方贞术调脂方对长期高脂饮食致非酒精性脂肪肝(NAFLD)小鼠的防治作用及肝脏内质网应激(ERS)的调节作用。方法:采用高脂纯化配方饲料喂养建立NAFLD小鼠模型,同时防治性给予复方贞术调脂方20 w,通过观察血浆、肝脏的总胆固醇(TC)和甘油三酯(TG)水平、肝组织病理形态学以及脂质代谢和ERS相关基因的表达,考察复方贞术调脂方对NAFLD的防治作用及机制。结果:与正常对照组比较,模型组血浆及肝脏组织中TC、TG含量及肝组织PERK mRNA表达显著升高(P<0.05);与模型组比较,复方贞术调脂方低剂量组血浆及肝组织中TC、TG含量均显著降低(P<0.05),肝组织XBP-1、PERK、SREBP-1c mRNA表达显著下调(P<0.05)。结论:复方贞术调脂方能显著缓解长期高脂饮食诱导的小鼠NAFLD形成,调节肝脏ERS可能是其作用途径之一。Objective： To investigate the effect of Fufang Zhenzhu Tiaozhi formula（ FZT） in a long-term high-fat diet-induced nonalcoholic fatty liver disease（ NAFLD） mouse model and to study the regulation of hepatic endoplasmic reticulum stress（ ERS）. Methods：Mice model with NAFLD was established by feeding purified high-fat diet,and treated with FZT at the same time. After treatment with FZT for 20 weeks,the plasma total cholesterol（ TC）,hepatic TG,triglyceride（ TG） level,liver tissue pathology morphology and expression of lipid metabolism,ERS related genes were observed,and measured the effect of FZT on NAFLD in mice. Results： Compared with normal control group,plasma and hepatic TC,TG level were significantly increased in model group（ P〈0. 05）; compared with model group,the plasma and hepatic TC,TG level were significantly lower in FZT high and low-dose group（ P〈0. 05）; XBP-1,PERK and SREBP-1c mRNA expression of liver tissue were significantly lower（ P〈0. 05）. Conclusion： FZT can significantly alleviate NAFLD in mice which induced by a long-term high-fat diet,reduction of the hepatic ERS activity may be one of its mechanisms alleviate NAFLD.

关 键 词：贞术调脂方 脂肪肝 内质网应激 

分 类 号：R285.5[医药卫生—中药学]