Bacillus amyloliquefaciens Strain W19 can Promote Growth and Yield and Suppress Fusarium Wilt in Banana Under Greenhouse and Field Conditions  

作　　者：WANG Beibei SHEN Zongzhuan ZHANG Fengge Waseem RAZA YUAN Jun HUANG Rong RUAN Yunze LI Rong SHEN Qirong 

机构地区：[1]National Engineering Research Center for Organic-Based Fertilizers, Jiangsu Key Lab and Engineering Center for Solid Organics Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University [2]Hainan Key Laboratory for Sustainable Utilization of Tropical Bio-Resources, College of Agriculture, Hainan University [3]Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University

出　　处：《Pedosphere》2016年第5期733-744,共12页土壤圈（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 31572212 and 31372142);the National Key Basic Research Program of China (No. 2015CB150503);the Chinese Ministry of Science and Technology (No. 2013AA102802);the Natural Science Foundation of Jiangsu Province, China (No. BK20150059);the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions of China;the 111 Project of China (No. B12009);the National Training Program of Innovation and Entrepreneurship for Undergraduates of China (No. 201410307089);the "Qing Lan" Project of China

摘　　要：Plant growth-promoting rhizobacteria （PGPR） are considered to be the most promising agents for cash crop production via increasing crop yields and decreasing disease occurrence. The Bacillus amyloliquefaciens strain W19 can produce secondary metabolites （iturin and bacillomycin D） effectively against Fusarium oxysporum f. sp. cubense （FOC）. In this study, the ability of a bio-organic fertilizer （BIO） containing strain W19 to promote plant growth and suppress the Fusarium wilt of banana was evaluated in both pot and field experiments. The results showed that application of BIO significantly promoted the growth and fruit yield of banana while suppressing the banana Fusariurn wilt disease. To further determine the beneficial mechanisms of the strain, the colonization of green fluorescent protein-tagged strain W19 on banana roots was observed using confocal laser scanning microscopy and scanning electron microscopy. The effect of banana root exudates on the formation of biofilm of strain W19 indicated that the banana root exudates may enhance colonization. In addition, the strain W19 was able to produce indole-3-acetic acid （IAA）, a plant growth-promoting hormone. The results of these experiments revealed that the application of strain W19-enriched BIO improved the banana root colonization of strain W19 and growth of banana and suppressed the Fusarium wilt. The PGPR strain W19 can be a useful biocontrol agent for the production of banana under field conditions.Plant growth-promoting rhizobacteria(PGPR) are considered to be the most promising agents for cash crop production via increasing crop yields and decreasing disease occurrence. The Bacillus amyloliquefaciens strain W19 can produce secondary metabolites(iturin and bacillomycin D) effectively against Fusarium oxysporum f. sp. cubense(FOC). In this study, the ability of a bio-organic fertilizer(BIO) containing strain W19 to promote plant growth and suppress the Fusarium wilt of banana was evaluated in both pot and field experiments. The results showed that application of BIO significantly promoted the growth and fruit yield of banana while suppressing the banana Fusarium wilt disease. To further determine the beneficial mechanisms of the strain, the colonization of green fluorescent protein-tagged strain W19 on banana roots was observed using confocal laser scanning microscopy and scanning electron microscopy. The effect of banana root exudates on the formation of biofilm of strain W19 indicated that the banana root exudates may enhance colonization. In addition, the strain W19 was able to produce indole-3-acetic acid(IAA), a plant growth-promoting hormone.The results of these experiments revealed that the application of strain W19-enriched BIO improved the banana root colonization of strain W19 and growth of banana and suppressed the Fusarium wilt. The PGPR strain W19 can be a useful biocontrol agent for the production of banana under field conditions.

关 键 词：BIOCONTROL bio-organic fertilizer COLONIZATION crop yield fungal disease indole-3-acetic acid plant growth-promoting rhizobacteria （PGPR） root exudates 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]