铸造工人PCDD／Fs接触水平与氧化损伤的关系  

The relationships of polychlorinated dibenzo-p-dioxins and dibenzofurans （PCDD/Fs） inhalational dose and oxidative damage in foundry workers

作　　者：何金铜 张素坤[2] 赵明[1] 张庄[1] 史廷明[3] 陈卫红[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,环境与健康教育部重点实验实验,武汉430030 [2]环境保护部华南环境科学研究所 [3]湖北省预防医学科学院

出　　处：《中华劳动卫生职业病杂志》2016年第9期647-652,共6页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的评估铸造生产过程产生的二噁英类化合物（PCDD／Fs）水平及其对机体氧化损伤的影响。方法选择某铸造厂工人为研究对象，以铸造厂辅助人员和神农架林区居民为对照，其中铸造工人105名，辅助人员104人，神农架林区居民100名；采用同位素稀释高分辨气相色谱-高分辨质谱法测定铸造厂作业点、厂区环境和神农架居民区环境空气中的PCDD／Fs，评估研究对象经呼吸摄入PCDD／Fs的含量。采用高效液相色谱一电化学检测器法测定尿8-羟基-2．脱氧鸟苷（8-OHdG）为DNA氧化损伤指标，Elisa试剂盒测定尿中8一异前列腺素F2ct（8-iso—PGF2a）水平作为脂质过氧化指标。结果铸造厂作业点、厂区和对照环境空气PCDD／Fs的毒性当量分别为（0．21±0．08）、（0．03±0．01）和（0．0012±0．0007）Pg·TEQ／m3。对应的三组人群经呼吸摄入PCDD／Fs的含量从高到低为：铸造厂工人92．54（32．09．264．49）、辅助工人17．053（13．09～25．13）、对照组0．46（0．32～0．77）fg·TEQ／kg·d，差异有统计学意义（P〈O．05）。铸造厂工人、辅助工人和对照居民尿中8-OHdG依次为3．25（1．07—15．36）、2．72（0．75—16．07）、2．17（0．38～17．07）nmol／L肌酐，差异有统计学意义（P〈．05）；尿中8-iso—PGF2a水平依次为19．11（9．33～76．50）、20．95（8．40～79．31）、11．48f4．85—37．80）nmol／L肌酐，差异有统计学意义（P〈0．05）。经过校正性别、年龄、吸烟等混杂因素后，尿中8-OHdG和8-iso—PGF2α含量随着PCDD／Fs四分位浓度升高而增加，呈现剂量反应关系，趋势检验，差异有统计学意义（P〈0．05）。结论环境空气暴露PCDD／Fs可导致体内DNA氧化损伤和脂质过氧化升高，呈现剂量效应关系。Objective To investigate the potential dose-response relationship between PCDD/Fs inhalational exposure and oxidative damage to DNA and lipid in foundry workers. Methods The participants were divided into three groups, including exposure group, administrator group and control group. The concentrations of polychlorinated dibenzo-p-dioxins and dibenzofurans （PCDD/Fs） in air environment were determined by isotope dilution high resolution capillary column gas chromatography/high resolution mass spectrometry （HRGC-HRMS）. The inhalational dose of PCDD/Fs were evaluated by Houmen＇s model. Urinary 8- OHdG was analyzed by high performance liquid chromatography-electrochemical detector（HPLC-ECD）. Urinary 8-iso-PGF2u were determined by ELISA. Results The PCDD/Fs environmental concentrations of three groups （pg.TEQ/m） were 0.21±0.08, 0.03 ±0.01, 0.0012±0.0007 （P〈0.05）, respectively. The inhalational dose of exposure group（92.54（32.90-264.49） fg＇TEQ/kg-d） was 5.4 folds higher than the administrator group, and was 201 times more than control group. The levels of urinary 8-OHdG（nmol/L crea） of three groups were 3.25 （1.07- 15.36）, 2.72（0.75-16.07）, 2.17（0.38-17.07） （P〈0.05） and the levels of urinary 8-iso-PGF2u （nmol/L crea） were 19.11 （9.33-76.50）, 20.95 （8.40-79.31）, 11.48 （4.85-37.80） （P〈0.05）, respectively. The multiple linear regression analysis revealed that there were significantly increased trend for levels of urinary 8-OHdG and 8-iso-PGF2α as PCDD/Fs exposure levels increased, after adjustment for age, sex, smoking statue, alcohol use and BMI （P〈0.01）. Conclusion There was a dose-response relationship between PCDD/Fs inhalational exposure and oxidative damage in foundry workers.

关 键 词：职业暴露 二噁英类化合物 8-羟基-2-脱氧鸟苷 8-异前列腺素F2Α 

分 类 号：R135[医药卫生—劳动卫生]