白纹伊蚊内源性登革病毒序列分析  

Analysis of endogenous viral elements derived from dengue virus in Aedes albopictus genome

作　　者：王衍海[1] 顾金保[2] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052 [2]南方医科大学公共卫生学院,广州510515

出　　处：《中华实验和临床病毒学杂志》2016年第4期337-339,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：基金项目：国家自然科学基金（81371846）;珠江科技新星（2014J2200032）

摘　　要：目的 分析和验证白纹伊蚊（佛山株）基因组中的登革病毒来源的内源性病毒序列（endogenous viral elements,EVEs）.方法 使用Blast序列比对工具,分别以四型登革病毒基因组全序列为待查序列对白纹伊蚊基因组进行序列比对分析.根据筛选得到的EVEs序列设计基因特异性引物,分别以雌、雄各8只成蚊个体进行基因组PCR检测并测序验证.结果 在白纹伊蚊基因组上共计找到5个与登革病毒高度同源性的EVEs片段.其中,EVE-1与DENV-2 P8-1407MS株、EVE-2-5与DENV-1 02-20株具有高度的相似性.基因组PCR检测结果显示,EVE-1在种群个体中保守存在,而EVE-2未检测到,EVE-3-5在不同个体中变异度较高.结论 白纹伊蚊基因组中存在登革病毒序列来源的EVEs,为研究登革病毒与媒介之间的相互作用提供了新的方向.Objective To identify and analyze dengue virus-derived endogenous viral elements （EVEs） in Aedes albopictus （Foshan strain）.Methods The full sequences of four serotypes of dengue virus （DENV 1-4） were used as queries,and Blast N program was used to scan the genome of Ae.albopictus.The candidates of EVEs were confirmed by PCR method using specific primers with genomic DNA as template from 8 individuals of female and male adult,respectivly.Results Total 5 EVEs candidates displayed the high genetic similarity with DENV genome sequences.Among them,EVE-1 showed 100% genetic similarity with DENV-2 P8-1407MS strain,whereas EVE-2-5 showed more than 99% genetic similarity with DENV-1 02-20 strain.Genomic DNA PCR results indicated that EVE-1 exhibit sequence conservation across individuals among population,whereas the EVE-3-5 displayed the genomic sequence variation in individuals.Conclusions Dengue virus-derived EVEs were indentified in Ae.albopictus,that may provide new insight into the interaction between DENV and their vectors.

关 键 词：登革热病毒 伊蚊属 内源性病毒序列 

分 类 号：R373[医药卫生—病原生物学]