流式磁珠PCR-SSOP方法HLA基因分型可疑结果的确认分析  

HLA genotyping on 197 cases with ambiguous results by PCR-SSOP

作　　者：康轶青[1] 刘铮[1] 张丽[1] 马茹[1] 杜鹃[1] 杨贺才[1] 胡迎春[1] 张伯伟[1] 

机构地区：[1]河南省红十字血液中心,河南郑州450000

出　　处：《中国输血杂志》2016年第7期756-758,共3页Chinese Journal of Blood Transfusion

摘　　要：目的对PCR-SSOP方法未明确HLA分型结果的可疑样本进一步分型,并分析原因。方法对采用PCRSSOP方法进行HLA分型出现的131例可疑样本采用LABScan3D分型方法进行检测。对于3D分型仍不能确定的样本采用PCR-SBT分型方法进行检测。结果 131例可疑样本分析,其中87例LABScan3D分型方法可确定分型结果,余下的44例采用PCR-SBT获得分型结果,3种分型方法检测结果在低分水平上是完全一致的。结论 LABScan3D分型方法在PCR-SSOP的基础上进一步发展,可代替其进行大量样本的HLA分型,但是仍有引物探针的局限,不能代替SBT的地位。Objective To analyze the samples which had uncertain HLA genotyping results by PCR-SSOP. Methods131 samples which had uncertain HLA genotyping results by PCR-SSOP were typed by LABScan3 D. Those remain to be undetermined by LABScan3 D were verified again by PCR-SBT. Results 131 samples had suspicious results in a total of 4 000 samples analyzed by PCR-SSOP in 2014,among which,87 samples could be determined by LABScan3 D,and the rest of 44 samples could be determined by PCR-SBT. Those three HLA genotyping methods showed a complete coincidence at a low resolution level. Conclusion LABScan3 D which further develops on the basis of PCR-SSOP has many advantages. It could be used for a large number of samples in HLA genotyping. However,LABScan3 D has a limited number of oligonucleotide probes,which could not occupy the position of PCR-SBT.

关 键 词：人类白细胞抗原 LABScan3D 多聚酶链反应序列特异性寡核苷酸探针杂交法 DNA直接测序法 

分 类 号：R457.11[医药卫生—治疗学]