苦豆草提取物对奶牛乳房炎致病菌的体外抑菌作用  

The antibacterial effect of sophora alopecuroides extract on mastitis pathogens in vitro

作　　者：叶文初[1] 袁富威 班贇 张洁[1] 张耀相[1] 刘宇[2] 郭文柱[2] 杨珍[2] 李引乾[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100 [2]中国农业科学院兰州畜牧与兽药研究所/农业部兽用药物创制重点实验室/甘肃省新兽药工程重点实验室,甘肃兰州730050

出　　处：《中国兽医学报》2016年第9期1568-1571,共4页Chinese Journal of Veterinary Science

基　　金："十二五"农村领域国家科技计划资助项目(2011AA10A214)

摘　　要：从陕西宝鸡某奶牛场患乳房炎的奶牛中采取奶样46份,用于分离与鉴定致奶牛乳房炎的病原菌。采用牛津杯法和试管二倍稀释法对引起奶牛乳房炎致病菌的体外抑菌作用。结果显示:本试验分离鉴定出金黄色葡萄球菌、停乳链球菌、表皮葡萄球菌及致病性大肠杆菌;苦豆草提取物对致奶牛乳房炎的病原菌(金黄色葡萄球菌、停乳链球菌、表皮葡萄球菌、致病性大肠杆菌)的最低抑菌质量浓度(MIC)分别为2.340、0.585、2.340、4.687mg/L,最低杀菌质量浓度(MBC)均为9.375mg/L。结果表明:苦豆草提取物在体外具有明显的抑菌作用。The purpose of this study was to evaluate the antibacterial effect of sophora alopecuroides extract on mastitis pathogens in vitro. Forty six sterile milk sample from Shaanxi Baoji in dairy mastitis cows were used to isolate and identify mastitis pathogenic bacteria. The anti-bacterial effect of sophora alopecuroides extracts were tested by Oxford cup and tube double dilution method in vitro. In 4 identified pathogenic bacteria,such as Str. dysgalactiae,S, aureus,S, epiderrnidis and E. coli, the minimum inhibition concentration （MIC） of sophora alopecuroides extracts for 4 bacteria was 2. 340,0. 585,2. 340 and 4. 687 mg/L, respectively. The minimal bactericidal concen- tration （MBC） of sophora alopecuroides extracts for 4 bacteria was 9. 375 g/L,indicating that sophora alopecuroides extracts had obvious bacteriostatic effect on 4 bacteria in vitro.

关 键 词：苦豆草提取物 体外抑菌 最小抑菌浓度 最小杀菌浓度 

分 类 号：S859[农业科学—临床兽医学]